PUM2 - Antibodies - The Human Protein Atlas

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PUM2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA030316

Antibody HPA049670

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA030316

HPA049670

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA030316, RRID:AB_10610289

Atlas Antibodies Cat#HPA049670, RRID:AB_2680845

VALIDATION SUMMARY

Antibody HPA030316

Antibody HPA049670

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA030316

Antibody HPA049670

Formal validation: Genetic

Validated

Significant downregulation > 25% by both siRNA:s.

Figure description

Cytoplasmic region of segmented cells in 10x-images

Antibody dilution

1:31

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane & cytosol.

Antibody dilution

1:31

1:77

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA030316

Antibody HPA049670

Standard validation

Approved

Figure description

Immunohistochemical staining of human thyroid gland shows distinct cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 72 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA030316

Antibody HPA049670

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

114, 107.6, 99.6, 94.1, 32

Antibody dilution

1:250

1:380

PROTEIN ARRAY

Antibody HPA030316

Antibody HPA049670

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:3050

ANTIGEN INFORMATION

Antibody HPA030316

Antibody HPA049670

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

ASPTEVVERLGPNTNPSEGLGPLPNPTANKPLVEEFSNPETQNLDAMEQV
GLESLQF

GTRDAETDGPEKGDQKGKASPFEEDQNRDLKQGDDDDSKINGRGLPNGMD
ADCKDFNRTPGSRQ

Matching transcripts

PUM2-001 - ENSP00000338173 [100%]
PUM2-002 - ENSP00000385992 [100%]
PUM2-003 - ENSP00000409905 [100%]
PUM2-006 - ENSP00000387399 [100%]
PUM2-201 - ENSP00000326746 [100%]
PUM2-202 - ENSP00000354370 [100%]

Other gene match

ANTIGEN VIEW

PUM2-001	PUM2-002	PUM2-003	PUM2-006	PUM2-201	PUM2-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.