Cell atlas - PUM2 - The Human Protein Atlas

GENERAL INFORMATION
Gene name	PUM2
Gene description	Pumilio RNA-binding family member 2
Protein class	Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	7
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Mainly localized to the cytosol.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Cytosol (validated)
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.
Mouse	Localized to the Cytosol in mouse cells.

DATA RELIABILITY
Reliability score	Validated based on 2 antibodies. HPA030316 , HPA049670
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Mainly localized to the cytosol.

Main location

Cytosol (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA030316	A-431	Cytosol
HPA030316	U-2 OS	Cytosol
HPA030316	U-251 MG	Cytosol
HPA049670	A-431	Nuclear membrane
HPA049670	A-431	Cytosol
HPA049670	U-2 OS	Cytosol
HPA049670	U-251 MG	Nuclear membrane
HPA049670	U-251 MG	Cytosol

siRNA VALIDATION

Antibody

HPA030316

siRNA validation

Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

Cytoplasmic region of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

71%

69%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Sample	Cell line
HPA030316	siRNA 1	U-2 OS
HPA030316	siRNA 2	U-2 OS
HPA030316	scrambled	U-2 OS

MOUSE CELLS

Main location

Cytosol

Mouse gene

ENSMUSG00000020594 (version 83)


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail	Antibody	Cell line	Location
	HPA049670	NIH 3T3	Cytosol

GENE INFORMATION
Gene name	PUM2 (HGNC Symbol)
Synonyms	KIAA0235, PUMH2
Description	Pumilio RNA-binding family member 2 (HGNC Symbol)
Entrez gene summary	This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Chromosome	2
Cytoband	p24.1
Chromosome location (bp)	20248691 - 20352234
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000055917 (version 83.38)
Entrez gene	23369
UniProt	Q8TB72 (UniProt - Evidence at protein level)
neXtProt	NX_Q8TB72
Antibodypedia	PUM2 antibodies

PROTEIN BROWSER

PUM2-001	PUM2-002	PUM2-003	PUM2-006	PUM2-009	PUM2-201	PUM2-202

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
PUM2-001 ENSP00000338173 ENST00000338086	Q8TB72 [Direct mapping] Pumilio homolog 2 Show all	Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0003723 [RNA binding] GO:0003730 [mRNA 3'-UTR binding] GO:0005488 [binding] GO:0005515 [protein binding] GO:0005737 [cytoplasm] GO:0006417 [regulation of translation] GO:0010494 [cytoplasmic stress granule] GO:0031965 [nuclear membrane] GO:0034063 [stress granule assembly] GO:0044822 [poly(A) RNA binding] GO:0048471 [perinuclear region of cytoplasm] GO:0060964 [regulation of gene silencing by miRNA] GO:1900246 [positive regulation of RIG-I signaling pathway] GO:2000637 [positive regulation of gene silencing by miRNA] Show all	1064 aa 114 kDa	No	0
PUM2-002 ENSP00000385992 ENST00000403432	Q8TB72 [Direct mapping] Pumilio homolog 2 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0003723 [RNA binding] GO:0003730 [mRNA 3'-UTR binding] GO:0005488 [binding] GO:0005515 [protein binding] GO:0005737 [cytoplasm] GO:0006417 [regulation of translation] GO:0010494 [cytoplasmic stress granule] GO:0031965 [nuclear membrane] GO:0044822 [poly(A) RNA binding] GO:0048471 [perinuclear region of cytoplasm] GO:0060964 [regulation of gene silencing by miRNA] GO:1900246 [positive regulation of RIG-I signaling pathway] GO:2000637 [positive regulation of gene silencing by miRNA] Show all	1064 aa 114 kDa	No	0
PUM2-003 ENSP00000409905 ENST00000440577	A0A0C4DG68 [Direct mapping] Pumilio homolog 2 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0003723 [RNA binding] GO:0005488 [binding] Show all	876 aa 94.1 kDa	No	0
PUM2-006 ENSP00000387399 ENST00000442400	C9JW01 [Direct mapping] Pumilio homolog 2 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005737 [cytoplasm] GO:0031965 [nuclear membrane] Show all	293 aa 32 kDa	No	0
PUM2-009 ENSP00000395098 ENST00000424110	C9JE24 [Direct mapping] Pumilio homolog 2 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		34 aa 3.9 kDa	No	0
PUM2-201 ENSP00000326746 ENST00000319801	A0A0A0MR59 [Direct mapping] Pumilio homolog 2 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0003723 [RNA binding] GO:0005488 [binding] Show all	929 aa 99.6 kDa	No	0
PUM2-202 ENSP00000354370 ENST00000361078	Q8TB72 [Direct mapping] Pumilio homolog 2 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0003723 [RNA binding] GO:0003730 [mRNA 3'-UTR binding] GO:0005488 [binding] GO:0005515 [protein binding] GO:0005737 [cytoplasm] GO:0006417 [regulation of translation] GO:0010494 [cytoplasmic stress granule] GO:0031965 [nuclear membrane] GO:0044822 [poly(A) RNA binding] GO:0048471 [perinuclear region of cytoplasm] GO:0060964 [regulation of gene silencing by miRNA] GO:1900246 [positive regulation of RIG-I signaling pathway] GO:2000637 [positive regulation of gene silencing by miRNA] Show all	1008 aa 107.6 kDa	No	0