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GYG2

ANTIBODY INFORMATION
 
 

Antibody HPA005495

  

Antibody HPA064686

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA005495   HPA064686  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA005495, RRID:AB_1079035   Atlas Antibodies Cat#HPA064686, RRID:AB_2685323  

VALIDATION SUMMARY
 
 

Antibody HPA005495

  

Antibody HPA064686

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA005495

  

Antibody HPA064686

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleus, nucleoli & cytosol.  

Antibody dilution

   1:200  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA005495

  

Antibody HPA064686

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in cells in tubules.   Immunohistochemical staining of human liver shows distinct cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 29 tissues
Protein: detected in 35 cell types		   RNA: detected in 29 tissues
Protein: detected in 19 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:550   1:2500  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA005495

  

Antibody HPA064686

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 55.2, 52, 47.5, 26.2   55.2, 52  

Antibody dilution

 1:250   1:860  

PROTEIN ARRAY
 
 

Antibody HPA005495

  

Antibody HPA064686

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:10300  

ANTIGEN INFORMATION
 
 

Antibody HPA005495

  

Antibody HPA064686

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 138   93  

Antigen sequence

 
PAFKQFGSSAKVVHFLGSMKPWNYKYNPQSGSVLEQGSASSSQHQAAFLH
LWWTVYQNNVLPLYKSVQAGEARASPGHTLCHSDVGGPCADSASGVGEPC
ENSTPSAGVPCANSPLGSNQPAQGLPEPTQIVDETLSL
  
MIACPETETPAVITCDPLSQPSPQPADFTETETILQPANKVESVSSEETF
EPSQELPAEALRDPSLQDALEVDLAVSVSQISIEEKVKELSPE
 

Matching transcripts

 GYG2-001 - ENSP00000370555 [100%]
GYG2-005 - ENSP00000487294 [100%]
GYG2-007 - ENSP00000381786 [100%]
GYG2-010 - ENSP00000370549 [100%]
  GYG2-001 - ENSP00000370555 [100%]
GYG2-007 - ENSP00000381786 [100%]
 

Other gene match

    
ANTIGEN VIEW
GYG2-001
GYG2-005
GYG2-007
GYG2-010
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.