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SFSWAP

ANTIBODY INFORMATION
 
 

Antibody HPA039362

  

Antibody HPA040063

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039362   HPA040063  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   8  

References

    

Proper citation

 Atlas Antibodies Cat#HPA039362, RRID:AB_10794089   Atlas Antibodies Cat#HPA040063, RRID:AB_10795413  

VALIDATION SUMMARY
 
 

Antibody HPA039362

  

Antibody HPA040063

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039362

  

Antibody HPA040063

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & plasma membrane.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.  

Antibody dilution

 1:200   1:154  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039362

  

Antibody HPA040063

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:150  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039362

  

Antibody HPA040063

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 110, 104.8   110, 104.8  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA039362

  

Antibody HPA040063

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:20000   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA039362

  

Antibody HPA040063

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 98   91  

Antigen sequence

 
NYLHPSLFASKKCNRLEELMKPLKVVDPDHPLAALVRKAQADSSTPTPHN
ADGAPVQPSQVEYTADSTVAAMYYSYYMLPDGTYCLAPPPPGIDVTTY
  
VELPPTAKMHAIIERTASFVCRQGAQFEIMLKAKQARNSQFDFLRFDHYL
NPYYKFIQKAMKEGRYTVLAENKSDEKKKSGVSSDNEDDDD
 

Matching transcripts

 SFSWAP-001 - ENSP00000261674 [100%]
SFSWAP-003 - ENSP00000437738 [100%]
  SFSWAP-001 - ENSP00000261674 [100%]
SFSWAP-003 - ENSP00000437738 [100%]
 

Other gene match

    
ANTIGEN VIEW
SFSWAP-001
SFSWAP-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.