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Immunofluorescent staining of human cell line SK-MEL-30 shows localization to plasma membrane.
Antibody dilution
1:135
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA043310
Antibody HPA076091
Antibody CAB017632
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human liver shows strong granular positivity in hepatocytes.
Expression
RNA: detected in 35 tissues Protein: detected in 31 cell types
RNA: detected in 35 tissues Protein: detected in 20 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:700
1:100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043310
Antibody HPA076091
Antibody CAB017632
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
98.1, 95.6, 95.2, 91.3, 72.2
98.1, 95.6, 95.2, 91.3, 72.2, 32
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA043310
Antibody HPA076091
Antibody CAB017632
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
