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ZNRD1
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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA055074

 

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA055074  

Host species

Rabbit  

Clonality

pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 

Released in version

12  

References

 

Proper citation

Atlas Antibodies Cat#HPA055074, RRID:AB_2682690  

VALIDATION SUMMARY

 
 

Antibody HPA055074

 
Immunocytochemistry

Approved  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY

 
 

Antibody HPA055074

 

Standard validation

Approved  

 

Figure description

Immunofluorescent staining of human cell line PC-3 shows localization to nucleoli fibrillar center.  

Antibody dilution

1:70  

Literature conformity

The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT

 
 

Antibody HPA055074

 

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
 

 

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
 

Target mass (kDa)

13.9  

Antibody dilution

1:350  

PROTEIN ARRAY

 
 

Antibody HPA055074

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
 

 

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

1:2800  

ANTIGEN INFORMATION

 
 

Antibody HPA055074

 

Antigen

Recombinant protein fragment  

Length (aa)

79  

Antigen sequence

CIRCGFNINVRDFEGKVVKTSVVFHQLGTAMPMSVEEGPECQGPVVDRRC
PRCGHEGMAYHTRQMRSADEGQTVFYTCT
 

Matching transcripts

ZNRD1-001 - ENSP00000352333 [100%]
ZNRD1-002 - ENSP00000331111 [100%]
ZNRD1-005 - ENSP00000365978 [100%]
ZNRD1-006 - ENSP00000365981 [100%]
 

Other gene match

 
ANTIGEN VIEW
ZNRD1-001
ZNRD1-002
ZNRD1-005
ZNRD1-006
 

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