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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane & cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli.
Antibody dilution
1:56
1:19
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA011165
Antibody CAB004573
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in nerve fibers.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 14 cell types
RNA: detected in 37 tissues Protein: detected in 12 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1250
1:500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011165
Antibody CAB004573
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402172)
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil