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GNB5

ANTIBODY INFORMATION
 
 

Antibody HPA041942

  

Antibody HPA044198

  

Antibody CAB032353

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA041942   HPA044198   sc-14947  

Host species

 Rabbit   Rabbit   Goat  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 8   12   6  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA041942, RRID:AB_10794309   Atlas Antibodies Cat#HPA044198, RRID:AB_2678853   Santa Cruz Biotechnology Cat#sc-14947, RRID:AB_2263274  

VALIDATION SUMMARY
 
 

Antibody HPA041942

  

Antibody HPA044198

  

Antibody CAB032353

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Uncertain     Uncertain  
Western blot

Uncertain   Uncertain   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041942

  

Antibody HPA044198

  

Antibody CAB032353

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.    

Antibody dilution

   1:96    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041942

  

Antibody HPA044198

  

Antibody CAB032353

  

Standard validation

 Uncertain     Uncertain  

     

Figure description

 Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuropil.     Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 51 cell types		     RNA: detected in 36 tissues
Protein: detected in 29 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:10     1:30  

Literature conformity

 Partly consistent with gene/protein characterization data.     Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041942

  

Antibody HPA044198

  

Antibody CAB032353

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 43.6   43.6, 38.8   43.6, 38.8, 31.2, 14.4  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA041942

  

Antibody HPA044198

  

Antibody CAB032353

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:30000    

ANTIGEN INFORMATION
 
 

Antibody HPA041942

  

Antibody HPA044198

  

Antibody CAB032353

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 107   51    

Antigen sequence

 
TFLVNVFGSCDKCFKQRALRPVFKKSQQLSYCSTCAEIMATEGLHENETL
ASLKSEAESLKGKLEEERAKLHDVELHQVAERVEALGQFVMKTRRTLKGH
GNKVLCM
  
DNKCSVYPLTFDKNENMAAKKKSVAMHTNYLSACSFTNSDMQILTASGDG
T
  

											 
																				

																		

										
											
Matching transcripts

												
																					GNB5-001 - ENSP00000261837 [100%] 

											 
																						GNB5-001 - ENSP00000261837 [100%] 
GNB5-002 - ENSP00000351635 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
GNB5-001
GNB5-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA041942

												
										 
																				
											
											
											
Antibody HPA044198

												
										 
																				
											
											
											
Antibody CAB032353

												
										 
																		

																	

										
											
1.

												
																						Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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