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FSTL3

ANTIBODY INFORMATION
 
 

Antibody HPA045378

  

Antibody CAB024899

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA045378   MAB1288  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 12   5  

References

    

Proper citation

 Atlas Antibodies Cat#HPA045378, RRID:AB_2679300    

VALIDATION SUMMARY
 
 

Antibody HPA045378

  

Antibody CAB024899

  
Immunocytochemistry

Supported    
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA045378

  

Antibody CAB024899

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.    

Antibody dilution

 1:24    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045378

  

Antibody CAB024899

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human hippocampus shows distinct nuclear and cytoplasmic positivity in neuronal cells and glial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 55 cell types		   RNA: detected in 37 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:25  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA045378

  

Antibody CAB024899

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 27.7   27.7, 10, 9.1, 4.2  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA045378

  

Antibody CAB024899

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA045378

  

Antibody CAB024899

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 75    

Antigen sequence

 
MGSGNPAPGGVCWLQQGQEATCSLVLQTDVTRAECCASGNIDTAWSNLTH
PGNKINLLGFLGLVHCLPCKDSCDG
   

Matching transcripts

 FSTL3-001 - ENSP00000166139 [100%]
   

Other gene match

    
ANTIGEN VIEW
FSTL3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.