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Immunofluorescent staining of human cell line CACO-2 shows localization to nucleus & nucleoli fibrillar center.
Antibody dilution
1:23
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA023690
Antibody HPA029634
Standard validation
Approved
Figure description
Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
Antibody dilution
1:10
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023690
Antibody HPA029634
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
127.5, 98.2
127.5, 98.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA023690
Antibody HPA029634
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
