GLI2 - Antibodies - The Human Protein Atlas

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GLI2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA074275

Antibody CAB008558

Provider

Atlas Antibodies
Sigma-Aldrich

Rockland

Product name

HPA074275

600-401-845

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA074275, RRID:AB_2686677

Rockland Cat#600-401-845, RRID:AB_2111910

VALIDATION SUMMARY

Antibody HPA074275

Antibody CAB008558

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA074275

Antibody CAB008558

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A549 shows localization to nucleus & nucleoli.

Antibody dilution

1:119

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA074275

Antibody CAB008558

Standard validation

Approved

Figure description

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and glial cells.

Expression

RNA: detected in 21 tissues
Protein: detected in 71 cell types

Retrieval

HIER pH6

Antibody dilution

1:1000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA074275

Antibody CAB008558

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.

Figure description

Target mass (kDa)

167.8, 57.2, 18.4, 8.5

Antibody dilution

1:500

PROTEIN ARRAY

Antibody HPA074275

Antibody CAB008558

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:4800

ANTIGEN INFORMATION

Antibody HPA074275

Antibody CAB008558

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

Antigen sequence

PNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMT
TMHRFEQLKKEKLKSLKDSCSWA

Matching transcripts

GLI2-009 - ENSP00000390436 [100%]
GLI2-201 - ENSP00000312694 [100%]
GLI2-202 - ENSP00000354586 [100%]

Other gene match

ANTIGEN VIEW

GLI2-009	GLI2-201	GLI2-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.