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CA12

ANTIBODY INFORMATION
 
 

Antibody HPA008773

  

Antibody HPA073203

  

Antibody CAB025181

  

Antibody CAB062549

  

Antibody CAB068179

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA008773   HPA073203   MAB2190   AMAb90639   AMAb90637  

Host species

 Rabbit   Rabbit   Mouse   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G   Protein A/G  

Other gene match

          

Released in version

 3   16   5   12   13  

References

 4          

Proper citation

 Atlas Antibodies Cat#HPA008773, RRID:AB_1078394   Atlas Antibodies Cat#HPA073203, RRID:AB_2686580     Atlas Antibodies Cat#AMAb90639, RRID:AB_2665617   Atlas Antibodies Cat#AMAb90637, RRID:AB_2665616  

VALIDATION SUMMARY
 
 

Antibody HPA008773

  

Antibody HPA073203

  

Antibody CAB025181

  

Antibody CAB062549

  

Antibody CAB068179

  
Immunocytochemistry

Approved   Approved        
Immunohistochemistry


Validated
    

Validated
  

Validated
  

Validated
  
Western blot

Supported   Uncertain   Uncertain   Uncertain   Supported  
Protein array

Supported   Approved        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008773

  

Antibody HPA073203

  

Antibody CAB025181

  

Antibody CAB062549

  

Antibody CAB068179

  

Standard validation

 Approved   Approved        

         

Figure description

 Immunofluorescent staining of human cell line RT4 shows localization to nucleus.   Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & vesicles.        

Antibody dilution

 1:25   1:91        

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008773

  

Antibody HPA073203

  

Antibody CAB025181

  

Antibody CAB062549

  

Antibody CAB068179

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		          

         

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.          
 

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

   
 
 
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported     Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.     Immunohistochemical staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in subset of cells in tubules.  

Expression

 RNA: detected in 27 tissues
Protein: detected in 40 cell types		     RNA: detected in 27 tissues
Protein: detected in 25 cell types		   RNA: detected in 27 tissues
Protein: detected in 24 cell types		   RNA: detected in 27 tissues
Protein: detected in 18 cell types		  

Retrieval

 HIER pH6     HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:75     1:90   1:250   1:8000  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.     Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA008773

  

Antibody HPA073203

  

Antibody CAB025181

  

Antibody CAB062549

  

Antibody CAB068179

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

         

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400487)		         Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 39.5, 38.4, 31.9   39.5, 38.4, 31.9   39.5, 38.4, 31.9   39.5, 38.4, 31.9   39.5, 38.4, 31.9  

Antibody dilution

 1:250   1:300   1:500   1:500   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA008773

  

Antibody HPA073203

  

Antibody CAB025181

  

Antibody CAB062549

  

Antibody CAB068179

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		        

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:3000   1:3650        

ANTIGEN INFORMATION
 
 

Antibody HPA008773

  

Antibody HPA073203

  

Antibody CAB025181

  

Antibody CAB062549

  

Antibody CAB068179

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein   Recombinant protein  

Length (aa)

 120   61        

Antigen sequence

 
TASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIE
ELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALY
CTHMDDPSPREMINNFRQVQ
  
NKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEH
TVSGQHFAAEL
  

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CA12-001 - ENSP00000178638 [100%] 
CA12-002 - ENSP00000343088 [100%] 
CA12-003 - ENSP00000403028 [100%] 

											 
																						CA12-001 - ENSP00000178638 [100%] 
CA12-002 - ENSP00000343088 [100%] 
CA12-003 - ENSP00000403028 [80%] 

											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CA12-001
CA12-002
CA12-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA008773

												
										 
																				
											
											
											
Antibody HPA073203

												
										 
																				
											
											
											
Antibody CAB025181

												
										 
																				
											
											
											
Antibody CAB062549

												
										 
																				
											
											
											
Antibody CAB068179

												
										 
																		

																	

										
											
1.

												
																						Immunophenotyping invasive breast cancer: paving the road for molecular imaging

	Vermeulen JF et al

	BMC Cancer 2012;12:240

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Gene expression signatures differentiate ovarian/peritoneal serous carcinoma from breast carcinoma in effusions

	Davidson B et al

	J Cell Mol Med 2011;15(3):535-44

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						Differential expression of growth factor receptors and membrane-bound tumor markers for imaging in male and female breast cancer

	Vermeulen JF et al

	PLoS One 2013;8(1):e53353

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
4.

												
																						Noninvasive detection of breast cancer lymph node metastasis using carbonic anhydrases IX and XII targeted imaging probes

	Tafreshi NK et al

	Clin Cancer Res 2012;18(1):207-19

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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by the Knut & Alice Wallenberg foundation.