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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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ACTN2
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    TISSUE

  • CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

ACTN2

Gene description

Actinin, alpha 2

Protein class

Disease related genes
Plasma proteins
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

3
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Tissue enhanced (head and neck cancer)
HPA (cell line):Cell line enhanced (BJ hTERT+ SV40 Large T+ RasG12V, HEK93, RH-30, SH-SY5Y, WM-115)
HPA (normal tissue):Group enriched (heart muscle, skeletal muscle)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic expression in striated muscle and brain.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data. Caution, targets protein from more than one gene.

Reliability score

Supported based on 2 antibodies.
HPA006035 , HPA008315
SHOW MORE

PROGNOSTIC SUMMARY

Gene product is not prognostic.
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Tissue enhanced (head and neck cancer)

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer

STAINING SUMMARY

HPA006035
HPA008315

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer tissues displayed moderate to strong cytoplasmic and membranous positivity. Several renal cancers were weakly stained or negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

All cancer tissues were negative.

GENE INFORMATION

Gene name

ACTN2

Synonyms

Description

Actinin, alpha 2 (HGNC Symbol)

Entrez gene summary

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

Chromosome

1

Cytoband

q43

Chromosome location (bp)

236686454 - 236764631

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000077522 (version 83.38)

Entrez gene

88

UniProt

P35609 (UniProt - Evidence at protein level)

neXtProt

NX_P35609

Antibodypedia

ACTN2 antibodies

PROTEIN BROWSER

ACTN2-001
ACTN2-201
ACTN2-202

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

ACTN2-001
ENSP00000355537
ENST00000366578
P35609 [Direct mapping]
Alpha-actinin-2
Show all
   THUMBUP predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000165 [MAPK cascade]
GO:0000186 [activation of MAPKK activity]
GO:0002576 [platelet degranulation]
GO:0005178 [integrin binding]
GO:0005509 [calcium ion binding]
GO:0005515 [protein binding]
GO:0005546 [phosphatidylinositol-4,5-bisphosphate binding]
GO:0005576 [extracellular region]
GO:0005829 [cytosol]
GO:0005856 [cytoskeleton]
GO:0005884 [actin filament]
GO:0005886 [plasma membrane]
GO:0005925 [focal adhesion]
GO:0007155 [cell adhesion]
GO:0007173 [epidermal growth factor receptor signaling pathway]
GO:0007264 [small GTPase mediated signal transduction]
GO:0007265 [Ras protein signal transduction]
GO:0007268 [synaptic transmission]
GO:0007411 [axon guidance]
GO:0007596 [blood coagulation]
GO:0008092 [cytoskeletal protein binding]
GO:0008286 [insulin receptor signaling pathway]
GO:0008307 [structural constituent of muscle]
GO:0008543 [fibroblast growth factor receptor signaling pathway]
GO:0019904 [protein domain specific binding]
GO:0030018 [Z disc]
GO:0030035 [microspike assembly]
GO:0030049 [muscle filament sliding]
GO:0030168 [platelet activation]
GO:0030175 [filopodium]
GO:0031093 [platelet alpha granule lumen]
GO:0031143 [pseudopodium]
GO:0031432 [titin binding]
GO:0038095 [Fc-epsilon receptor signaling pathway]
GO:0042391 [regulation of membrane potential]
GO:0042802 [identical protein binding]
GO:0042803 [protein homodimerization activity]
GO:0042981 [regulation of apoptotic process]
GO:0043197 [dendritic spine]
GO:0043267 [negative regulation of potassium ion transport]
GO:0043268 [positive regulation of potassium ion transport]
GO:0044325 [ion channel binding]
GO:0045087 [innate immune response]
GO:0045214 [sarcomere organization]
GO:0046983 [protein dimerization activity]
GO:0048010 [vascular endothelial growth factor receptor signaling pathway]
GO:0048011 [neurotrophin TRK receptor signaling pathway]
GO:0048041 [focal adhesion assembly]
GO:0051289 [protein homotetramerization]
GO:0051373 [FATZ binding]
GO:0051695 [actin filament uncapping]
GO:0070062 [extracellular exosome]
GO:0070080 [titin Z domain binding]
GO:0086097 [phospholipase C-activating angiotensin-activated signaling pathway]
GO:0090002 [establishment of protein localization to plasma membrane]
GO:1901017 [negative regulation of potassium ion transmembrane transporter activity]
GO:1901018 [positive regulation of potassium ion transmembrane transporter activity]
GO:2000009 [negative regulation of protein localization to cell surface]
GO:2001137 [positive regulation of endocytic recycling]
GO:2001259 [positive regulation of cation channel activity]
Show all
894 aa
103.9 kDa
No 0
ACTN2-201
ENSP00000443495
ENST00000542672
P35609 [Direct mapping]
Alpha-actinin-2
Show all
   MEMSAT3 predicted membrane proteins
   THUMBUP predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
Disease related genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000165 [MAPK cascade]
GO:0000186 [activation of MAPKK activity]
GO:0002576 [platelet degranulation]
GO:0005178 [integrin binding]
GO:0005509 [calcium ion binding]
GO:0005515 [protein binding]
GO:0005546 [phosphatidylinositol-4,5-bisphosphate binding]
GO:0005576 [extracellular region]
GO:0005829 [cytosol]
GO:0005856 [cytoskeleton]
GO:0005884 [actin filament]
GO:0005886 [plasma membrane]
GO:0005925 [focal adhesion]
GO:0006936 [muscle contraction]
GO:0007155 [cell adhesion]
GO:0007173 [epidermal growth factor receptor signaling pathway]
GO:0007264 [small GTPase mediated signal transduction]
GO:0007265 [Ras protein signal transduction]
GO:0007268 [synaptic transmission]
GO:0007411 [axon guidance]
GO:0007596 [blood coagulation]
GO:0008092 [cytoskeletal protein binding]
GO:0008286 [insulin receptor signaling pathway]
GO:0008307 [structural constituent of muscle]
GO:0008543 [fibroblast growth factor receptor signaling pathway]
GO:0019904 [protein domain specific binding]
GO:0030017 [sarcomere]
GO:0030018 [Z disc]
GO:0030035 [microspike assembly]
GO:0030049 [muscle filament sliding]
GO:0030168 [platelet activation]
GO:0030175 [filopodium]
GO:0030274 [LIM domain binding]
GO:0030374 [ligand-dependent nuclear receptor transcription coactivator activity]
GO:0030375 [thyroid hormone receptor coactivator activity]
GO:0030864 [cortical actin cytoskeleton]
GO:0031093 [platelet alpha granule lumen]
GO:0031143 [pseudopodium]
GO:0031432 [titin binding]
GO:0038095 [Fc-epsilon receptor signaling pathway]
GO:0042391 [regulation of membrane potential]
GO:0042802 [identical protein binding]
GO:0042803 [protein homodimerization activity]
GO:0042981 [regulation of apoptotic process]
GO:0043197 [dendritic spine]
GO:0043267 [negative regulation of potassium ion transport]
GO:0043268 [positive regulation of potassium ion transport]
GO:0044325 [ion channel binding]
GO:0045087 [innate immune response]
GO:0045214 [sarcomere organization]
GO:0046983 [protein dimerization activity]
GO:0048010 [vascular endothelial growth factor receptor signaling pathway]
GO:0048011 [neurotrophin TRK receptor signaling pathway]
GO:0048041 [focal adhesion assembly]
GO:0051289 [protein homotetramerization]
GO:0051373 [FATZ binding]
GO:0051695 [actin filament uncapping]
GO:0055013 [cardiac muscle cell development]
GO:0070062 [extracellular exosome]
GO:0070080 [titin Z domain binding]
GO:0086097 [phospholipase C-activating angiotensin-activated signaling pathway]
GO:0090002 [establishment of protein localization to plasma membrane]
GO:1901017 [negative regulation of potassium ion transmembrane transporter activity]
GO:1901018 [positive regulation of potassium ion transmembrane transporter activity]
GO:1903506 [regulation of nucleic acid-templated transcription]
GO:2000009 [negative regulation of protein localization to cell surface]
GO:2000273 [positive regulation of receptor activity]
GO:2001137 [positive regulation of endocytic recycling]
GO:2001259 [positive regulation of cation channel activity]
Show all
894 aa
103.9 kDa
No 0
ACTN2-202
ENSP00000438384
ENST00000546208
F6THM6 [Direct mapping]
Alpha-actinin-2
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005509 [calcium ion binding]
GO:0005515 [protein binding]
Show all
686 aa
79.7 kDa
No 0

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The Human Protein Atlas project is funded
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