We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nucleoli & the Golgi apparatus.
Antibody dilution
1:145
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA031976
Antibody CAB032500
Antibody CAB037291
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells and endothelial cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in endothelial cells and glandular cells.
Immunohistochemical staining of human colon shows plasma positivity in blood vessels.
Expression
RNA: detected in 36 tissues Protein: detected in 63 cell types
RNA: detected in 36 tissues Protein: detected in 60 cell types
RNA: detected in 36 tissues Protein: detected in 59 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:200
1:35
1:6000
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031976
Antibody CAB032500
Antibody CAB037291
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400720)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
24.4
24.4
24.4
Antibody dilution
1:250
1:500
1:500
PROTEIN ARRAY
Antibody HPA031976
Antibody CAB032500
Antibody CAB037291
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
