SCGN - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

SCGN

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA006641

Antibody CAB004005

Antibody CAB044060

Antibody CAB062563

Antibody CAB068232

Provider

Atlas Antibodies
Sigma-Aldrich

Medical University of Vienna

Atlas Antibodies
Sigma-Aldrich

Product name

HPA006641

scgn

SCGN 13B8

AMAB90630

AMAb90632

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA006641, RRID:AB_1079874

Atlas Antibodies Cat#AMAb90630, RRID:AB_2665612

Atlas Antibodies Cat#AMAb90632, RRID:AB_2665613

VALIDATION SUMMARY

Antibody HPA006641

Antibody CAB004005

Antibody CAB044060

Antibody CAB062563

Antibody CAB068232

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA006641

Antibody CAB004005

Antibody CAB044060

Antibody CAB062563

Antibody CAB068232

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human pancreas shows strong cytoplasmic and nuclear positivity in islets of Langerhans.

Immunohistochemical staining of human pancreas shows strong cytoplasmic and nuclear positivity in islet cells.

Immunohistochemical staining of human pancreas shows moderate cytoplasmic and nuclear positivity in islets of Langerhans.

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.

Expression

RNA: detected in 22 tissues
Protein: detected in 14 cell types

RNA: detected in 22 tissues
Protein: detected in 16 cell types

RNA: detected in 22 tissues
Protein: detected in 8 cell types

RNA: detected in 22 tissues
Protein: detected in 13 cell types

Retrieval

HIER pH6

Antibody dilution

1:500

1:12000

1:2000

1:4000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

Standard validation

Supported

Figure description

Staining of several subsets of neurons and their processes.

WESTERN BLOT

Antibody HPA006641

Antibody CAB004005

Antibody CAB044060

Antibody CAB062563

Antibody CAB068232

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

32, 5.6

Antibody dilution

1:250

1:500

1:1000

PROTEIN ARRAY

Antibody HPA006641

Antibody CAB004005

Antibody CAB044060

Antibody CAB062563

Antibody CAB068232

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA006641

Antibody CAB004005

Antibody CAB044060

Antibody CAB062563

Antibody CAB068232

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

139

Antigen sequence

RDLFLHHKKAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQENF
LLQFKMDACSTEERKRDFEKIFAYYDVSKTGALEGPEVDGFVKDMMELVQ
PSISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLK

Matching transcripts

SCGN-001 - ENSP00000367197 [100%]

Other gene match

ANTIGEN VIEW

SCGN-001

RELEVANT PUBLICATIONS

Antibody HPA006641

Antibody CAB004005

Antibody CAB044060

Antibody CAB062563

Antibody CAB068232

Tissue profiling of the mammalian central nervous system using human antibody-based proteomics
Mulder J et al
Mol Cell Proteomics 2009;8(7):1612-22

Secretagogin is a Ca2+-binding protein identifying prospective extended amygdala neurons in the developing mammalian telencephalon
Mulder J et al
Eur J Neurosci 2010;31(12):2166-77

Secretagogin is a Ca2+-binding protein specifying subpopulations of telencephalic neurons
Mulder J et al
Proc Natl Acad Sci U S A 2009;106(52):22492-7

Novel pancreatic beta cell-specific proteins: antibody-based proteomics for identification of new biomarker candidates
Lindskog C et al
J Proteomics 2012;75(9):2611-20

Clusters of secretagogin-expressing neurons in the aged human olfactory tract lack terminal differentiation
Attems J et al
Proc Natl Acad Sci U S A 2012;109(16):6259-64

The renaissance of Ca2+-binding proteins in the nervous system: secretagogin takes center stage
Alpár A et al
Cell Signal 2012;24(2):378-87

Neuronal calcium-binding proteins 1/2 localize to dorsal root ganglia and excitatory spinal neurons and are regulated by nerve injury
Zhang MD et al
Proc Natl Acad Sci U S A 2014;111(12):E1149-58

Distribution of secretagogin-containing neurons in the basal forebrain of mice, with special reference to the cholinergic corticopetal system
Gyengesi E et al
Brain Res Bull 2013;94:1-8
Application: IHC

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.