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Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center and non-germinal center cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra and reaction center cells.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.
Expression
RNA: detected in 37 tissues Protein: detected in 10 cell types
RNA: detected in 37 tissues Protein: detected in 9 cell types
RNA: detected in 37 tissues Protein: detected in 9 cell types
RNA: detected in 37 tissues Protein: detected in 7 cell types
RNA: detected in 37 tissues Protein: detected in 10 cell types
Retrieval
HIER pH6
HIER pH9
None
HIER pH6
HIER pH6
Antibody dilution
1:200
1:500
1:1
1:1000
1:4000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000440
Antibody CAB000052
Antibody CAB002800
Antibody CAB056154
Antibody CAB068221
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5 Lane 2: RT4 Lane 3: EFO-21 Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
147.5, 131.1, 83, 67.9
147.5, 131.1, 83, 67.9, 16.1, 12.6, 9.7, 4
147.5, 131.1, 83, 67.9, 16.1, 12.6, 9.7, 4
147.5, 131.1, 83, 67.9, 16.1, 12.6, 9.7, 4
147.5, 131.1, 83, 67.9, 16.1, 12.6, 9.7, 4
Antibody dilution
1:500
1:600
1:2
1:1000
1:1000
PROTEIN ARRAY
Antibody HPA000440
Antibody CAB000052
Antibody CAB002800
Antibody CAB056154
Antibody CAB068221
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
