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AKR1B1

ANTIBODY INFORMATION
 
 

Antibody HPA026425

  

Antibody HPA052751

  

Antibody CAB027391

  

Antibody CAB047353

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   NCI-CPTC
   NCI-CPTC
  

Product name

 HPA026425   HPA052751   CPTC-AKR1B1-3   CPTC-AKR1B1-2  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

        

Released in version

 6   12   6   12  

References

        

Proper citation

 Atlas Antibodies Cat#HPA026425, RRID:AB_10601563   Atlas Antibodies Cat#HPA052751, RRID:AB_2681935      

VALIDATION SUMMARY
 
 

Antibody HPA026425

  

Antibody HPA052751

  

Antibody CAB027391

  

Antibody CAB047353

  
Immunocytochemistry

Supported     Supported   Approved  
Immunohistochemistry


Validated
   Supported  

Validated
  

Validated
  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Approved   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA026425

  

Antibody HPA052751

  

Antibody CAB027391

  

Antibody CAB047353

  

Standard validation

 Supported     Supported   Approved  

       

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.     Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.  

Antibody dilution

 1:50     1:400   1:2000  

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA026425

  

Antibody HPA052751

  

Antibody CAB027391

  

Antibody CAB047353

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.   Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.   Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.   Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 7 cell types		   RNA: detected in 37 tissues
Protein: detected in 15 cell types		   RNA: detected in 37 tissues
Protein: detected in 5 cell types		   RNA: detected in 37 tissues
Protein: detected in 5 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:3500   1:700   1:45000   1:40000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA026425

  

Antibody HPA052751

  

Antibody CAB027391

  

Antibody CAB047353

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 35.9   35.9   35.9   35.9  

Antibody dilution

 1:250   1:80   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA026425

  

Antibody HPA052751

  

Antibody CAB027391

  

Antibody CAB047353

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:650      

ANTIGEN INFORMATION
 
 

Antibody HPA026425

  

Antibody HPA052751

  

Antibody CAB027391

  

Antibody CAB047353

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 51   46      

Antigen sequence

 
CTYHEKGLVKGACQKTLSDLKLDYLDLYLIHWPTGFKPGKEFFPLDESGN
V
  
VTPERIAENFKVFDFELSSQDMTTLLSYNRNWRVCALLSCTSHKDY
     

Matching transcripts

 AKR1B1-001 - ENSP00000285930 [100%]
  AKR1B1-001 - ENSP00000285930 [100%]
     

Other gene match

        
ANTIGEN VIEW
AKR1B1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.