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CTTN

ANTIBODY INFORMATION
 
 

Antibody HPA057242

  

Antibody CAB011235

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA057242   2190.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 13   3  

References

    

Proper citation

 Atlas Antibodies Cat#HPA057242, RRID:AB_2683379    

VALIDATION SUMMARY
 
 

Antibody HPA057242

  

Antibody CAB011235

  
Immunocytochemistry

Supported    
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA057242

  

Antibody CAB011235

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.    

Antibody dilution

 1:33    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA057242

  

Antibody CAB011235

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human stomach, lower shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 70 cell types		   RNA: detected in 37 tissues
Protein: detected in 77 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:115   1:500  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA057242

  

Antibody CAB011235

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 61.6   71, 61.6, 57.5, 29.6  

Antibody dilution

 1:110   1:500  

PROTEIN ARRAY
 
 

Antibody HPA057242

  

Antibody CAB011235

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1200    

ANTIGEN INFORMATION
 
 

Antibody HPA057242

  

Antibody CAB011235

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 76    

Antigen sequence

 
GFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYKTGFGGKFGVQSERQ
DSAAVGFDYKEKLAKHESQQDYSKGF
   

Matching transcripts

 CTTN-002 - ENSP00000301843 [100%]
   

Other gene match

    
ANTIGEN VIEW
CTTN-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.