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Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.
Immunohistochemical staining of human bone marrow shows strong positivity in hematopoietic cells.
Expression
RNA: detected in 24 tissues Protein: detected in 15 cell types
RNA: detected in 24 tissues Protein: detected in 45 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:800
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011041
Antibody CAB008370
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419723)
