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Term
Gene name

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Method

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Method

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Antibodies

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CEACAM6
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    TISSUE

  • CELL

  • PATHOLOGY

ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA011041

 

Antibody CAB008370

 

Provider

Atlas Antibodies
Sigma-Aldrich
  MBL
 

Product name

HPA011041   D028-3  

Host species

Rabbit   Mouse  

Clonality

pAb   mAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

CEACAM1 - ENSG00000079385 [94%]
CEACAM5 - ENSG00000105388 [82%]
CEACAM3 - ENSG00000170956 [100%]
AC011513.3 - ENSG00000267881 [82%]
   

Released in version

3   3  

References

   

Proper citation

Atlas Antibodies Cat#HPA011041, RRID:AB_1078481   MBL International Cat#D028-3, RRID:AB_592067  

VALIDATION SUMMARY

 
 

Antibody HPA011041

 

Antibody CAB008370

 
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA011041

 

Antibody CAB008370

 

Standard validation

Supported   Supported  

   

Figure description

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.   Immunohistochemical staining of human bone marrow shows strong positivity in hematopoietic cells.  

Expression

RNA: detected in 24 tissues
Protein: detected in 15 cell types
  RNA: detected in 24 tissues
Protein: detected in 45 cell types
 

Retrieval

HIER pH6   HIER pH6  

Antibody dilution

1:100   1:800  

Literature conformity

Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT

 
 

Antibody HPA011041

 

Antibody CAB008370

 

Standard validation

Supported

Band of predicted size in kDa (+/-20%) with additional bands present.
  Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.
 

   

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419723)
   

Target mass (kDa)

76.8, 76.7, 57.6, 57.2, 50.5, 50.3, 46.9, 43.1, 39.9, 37.7, 37.2, 27.1, 22.8, 21.7, 17.1, 15.6, 11, 10.9   37.2, 3.7  

Antibody dilution

1:250   1:500  

PROTEIN ARRAY

 
 

Antibody HPA011041

 

Antibody CAB008370

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
   

   

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

1:500    

ANTIGEN INFORMATION

 
 

Antibody HPA011041

 

Antibody CAB008370

 

Antigen

Recombinant protein fragment   Not known  

Length (aa)

33    

Antigen sequence

NVTQNDIGFYTLQVIKSDLVNEEATGQFHVYQE
   

Matching transcripts

CEACAM6-001 - ENSP00000199764 [94%]
   

Other gene match

CEACAM1 - ENSG00000079385 [94%]
CEACAM5 - ENSG00000105388 [82%]
CEACAM3 - ENSG00000170956 [100%]
AC011513.3 - ENSG00000267881 [82%]
   
ANTIGEN VIEW
CEACAM6-001
 

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