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Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.
Immunohistochemical staining of human appendix shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells. Microvilli also displayed strong immunoreactivity.
Expression
RNA: detected in 21 tissues Protein: detected in 15 cell types
RNA: detected in 21 tissues Protein: detected in 7 cell types
RNA: detected in 21 tissues Protein: detected in 15 cell types
RNA: detected in 21 tissues Protein: detected in 25 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH9
HIER pH9
Antibody dilution
1:100
1:20
1:400
1:5000
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011041
Antibody HPA019758
Antibody CAB000021
Antibody CAB000022
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419723)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
