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Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:10
1:75
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA001939
Antibody CAB002788
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human endometrium shows moderate cytoplasmic positivity in stromal cells.
Expression
RNA: detected in 36 tissues Protein: detected in 66 cell types
RNA: detected in 36 tissues Protein: detected in 19 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:15
1:300
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001939
Antibody CAB002788
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417923)
Target mass (kDa)
73.9, 68.8, 65.8, 28.3
73.9, 68.8, 65.8, 28.3, 22.9, 13.6
Antibody dilution
1:125
1:5000
PROTEIN ARRAY
Antibody HPA001939
Antibody CAB002788
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
