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SIGLEC1

ANTIBODY INFORMATION
 
 

Antibody HPA053457

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA053457  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 11  

References

  

Proper citation

 Atlas Antibodies Cat#HPA053457, RRID:AB_2682158  

VALIDATION SUMMARY
 
 

Antibody HPA053457

  
Immunohistochemistry

Supported  
Western blot

Supported  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA053457

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human lung shows distinct membranous positivity in macrophages.  

Expression

 RNA: detected in 32 tissues
Protein: detected in 10 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:250  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA053457

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 182.6, 48.4  

Antibody dilution

 1:530  

PROTEIN ARRAY
 
 

Antibody HPA053457

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:4250  

ANTIGEN INFORMATION
 
 

Antibody HPA053457

  

Antigen

 Recombinant protein fragment  

Length (aa)

 86  

Antigen sequence

 
CTAQNLLGSISTIGRLQVEGARVVAEPGLDVPEGAALNLSCRLLGGPGPV
GNSTFAWFWNDRRLHAEPVPTLAFTHVARAQAGMYH
 

Matching transcripts

 SIGLEC1-001 - ENSP00000341141 [100%]
SIGLEC1-002 - ENSP00000395778 [100%]
 

Other gene match

  
ANTIGEN VIEW
SIGLEC1-001
SIGLEC1-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.