Fields »
ABOUT
HELP
BLOG
TF

ANTIBODY INFORMATION
 
 

Antibody HPA001527

  

Antibody HPA005692

  

Antibody CAB009538

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA001527   HPA005692   sc-21011  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 2   3   4  

References

 1   1    

Proper citation

 Atlas Antibodies Cat#HPA001527, RRID:AB_1080357   Atlas Antibodies Cat#HPA005692, RRID:AB_1080354   Santa Cruz Biotechnology Cat#sc-21011, RRID:AB_793365  

VALIDATION SUMMARY
 
 

Antibody HPA001527

  

Antibody HPA005692

  

Antibody CAB009538

  
Immunocytochemistry

Approved   Approved    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001527

  

Antibody HPA005692

  

Antibody CAB009538

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus & mitochondria.   Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.    

Antibody dilution

 1:9   1:8    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001527

  

Antibody HPA005692

  

Antibody CAB009538

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 24 tissues
Protein: detected in 56 cell types		   RNA: detected in 24 tissues
Protein: detected in 18 cell types		   RNA: detected in 24 tissues
Protein: detected in 17 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:1500   1:600  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001527

  

Antibody HPA005692

  

Antibody CAB009538

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 77.1   77.1   77.1, 14.7, 8.1  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001527

  

Antibody HPA005692

  

Antibody CAB009538

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA001527

  

Antibody HPA005692

  

Antibody CAB009538

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 117   135    

Antigen sequence

 
DGPSVACVKKASYLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVA
EFYGSKEDPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPI
GLLYCDLPEPRKPLEKA
  
KDLLFKDSAHGFLKVPPRMDAKMYLGYEYVTAIRNLREGTCPEAPTDECK
PVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMS
LDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAG
  

											 
																				

																		

										
											
Matching transcripts

												
																					TF-001 - ENSP00000385834 [100%] 

											 
																						TF-001 - ENSP00000385834 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TF-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001527

												
										 
																				
											
											
											
Antibody HPA005692

												
										 
																				
											
											
											
Antibody CAB009538

												
										 
																		

																	

										
											
1.

												
																						Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model

	Kato BS et al

	Proteome Sci 2011;9:73

			
											 
																							Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model

	Kato BS et al

	Proteome Sci 2011;9:73

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.