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Immunohistochemical staining of human liver shows moderate cytoplasmic positivity, with a granular pattern, in hepatocytes.
Immunohistochemical staining of human kidney shows extra-cellular positivity in blood vessels and strong granular cytoplasmic positivity in cells of renal tubules.
Immunohistochemical staining of human lung shows distinct positivity in blood.
Expression
RNA: detected in 12 tissues Protein: detected in 30 cell types
RNA: detected in 12 tissues Protein: detected in 26 cell types
RNA: detected in 12 tissues Protein: detected in 12 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:100
1:250
1:125
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA001654
Antibody HPA003732
Antibody CAB022214
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424960)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424960)
Target mass (kDa)
38.3
38.3, 22.2, 19.9
38.3, 22.2, 19.9, 11.6
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA001654
Antibody HPA003732
Antibody CAB022214
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
