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Immunohistochemical staining of human testis shows moderate nuclear membranous positivity in cells in seminiferous ducts.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subsets of leukocytes.
Immunohistochemical staining of human duodenum shows distinct positivity in mast cells.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subset of non-germinal center cells.
Expression
RNA: detected in 24 tissues Protein: detected in 7 cell types
RNA: detected in 24 tissues Protein: detected in 18 cell types
RNA: detected in 24 tissues Protein: detected in 3 cell types
RNA: detected in 24 tissues Protein: detected in 5 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1400
1:450
1:200
1:500
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040182
Antibody HPA049554
Antibody CAB002215
Antibody CAB032871
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
26.6, 25.8
31.4, 31.3, 30.7, 30.5, 26.6, 25.8
26.6, 25.8
26.6, 25.8
Antibody dilution
1:250
1:300
1:500
1:500
PROTEIN ARRAY
Antibody HPA040182
Antibody HPA049554
Antibody CAB002215
Antibody CAB032871
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
