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Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subsets of leukocytes.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subsets of leukocytes.
Immunohistochemical staining of human lymph node shows distinct cytoplasmic positivity in subsets of cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of lymphoid cells outside reaction centra.
Expression
RNA: detected in 35 tissues Protein: detected in 11 cell types
RNA: detected in 35 tissues Protein: detected in 18 cell types
RNA: detected in 35 tissues Protein: detected in 11 cell types
RNA: detected in 35 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:600
1:450
1:4000
1:100000
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA049153
Antibody HPA049554
Antibody CAB016369
Antibody CAB022175
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
31.4, 31.3, 30.7, 30.5, 23.9
31.4, 31.3, 30.7, 30.5, 26.6, 25.8
31.3, 30.5, 23.9
31.3, 30.5, 23.9
Antibody dilution
1:100
1:300
1:500
1:500
PROTEIN ARRAY
Antibody HPA049153
Antibody HPA049554
Antibody CAB016369
Antibody CAB022175
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
