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Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subsets of leukocytes.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subsets of leukocytes.
Expression
RNA: detected in 32 tissues Protein: detected in 11 cell types
RNA: detected in 32 tissues Protein: detected in 18 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:600
1:450
Literature conformity
Consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA049153
Antibody HPA049554
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
31.4, 31.3, 30.7, 30.5, 23.9
31.4, 31.3, 30.7, 30.5, 26.6, 25.8
Antibody dilution
1:100
1:300
PROTEIN ARRAY
Antibody HPA049153
Antibody HPA049554
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
