Cell atlas - PHF5A - The Human Protein Atlas

GENERAL INFORMATION
Gene name	PHF5A
Gene description	PHD finger protein 5A
Protein class	Predicted intracellular proteins Transcription factors
Predicted localization	Intracellular
Number of transcripts	1
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the nucleoplasm.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleoplasm (validated)
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY
Reliability score	Validated based on 1 antibody. HPA028885
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the nucleoplasm.

Main location

Nucleoplasm (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA028885	A-431	Nucleoplasm
HPA028885	U-2 OS	Nucleoplasm
HPA028885	U-251 MG	Nucleoplasm

siRNA VALIDATION

Antibody

siRNA validation

Validated: Significant downregulation > 25% by one siRNA.

Analysis performed in

Nuclear region of segmented cells in 10x-images

siRNA 1

RFI

65%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail 10x	Thumbnail 40x	Antibody	Sample	Cell line
		HPA028885	scrambled	U-2 OS
		HPA028885	siRNA 1	U-2 OS

GENE INFORMATION
Gene name	PHF5A (HGNC Symbol)
Synonyms	bK223H9.2, INI, MGC1346, Rds3, SAP14b, SF3b14b, SF3B7
Description	PHD finger protein 5A (HGNC Symbol)
Entrez gene summary	This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes. [provided by RefSeq, Jul 2008]
Chromosome	22
Cytoband	q13.2
Chromosome location (bp)	41459717 - 41468725
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000100410 (version 83.38)
Entrez gene	84844
UniProt	Q7RTV0 (UniProt - Evidence at protein level)
neXtProt	NX_Q7RTV0
Antibodypedia	PHF5A antibodies

PROTEIN BROWSER

PHF5A-001

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
PHF5A-001 ENSP00000216252 ENST00000216252	Q7RTV0 [Direct mapping] PHD finger-like domain-containing protein 5A A0A024R1U2 [Target identity:100%; Query identity:100%] PHD finger protein 5A, isoform CRA_a Show all	Predicted intracellular proteins Transcription factors Yet undefined DNA-binding domains Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000398 [mRNA splicing, via spliceosome] GO:0003677 [DNA binding] GO:0003700 [transcription factor activity, sequence-specific DNA binding] GO:0005515 [protein binding] GO:0005654 [nucleoplasm] GO:0005686 [U2 snRNP] GO:0005689 [U12-type spliceosomal complex] GO:0006351 [transcription, DNA-templated] GO:0008380 [RNA splicing] GO:0010467 [gene expression] GO:0016363 [nuclear matrix] GO:0016607 [nuclear speck] GO:0044822 [poly(A) RNA binding] GO:0045893 [positive regulation of transcription, DNA-templated] GO:0071011 [precatalytic spliceosome] GO:0071013 [catalytic step 2 spliceosome] Show all	110 aa 12.4 kDa	No	0