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ANTIBODY INFORMATION
 
 

Antibody HPA000962

  

Antibody HPA004119

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA000962   HPA004119  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 1   2  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA000962, RRID:AB_1079722   Atlas Antibodies Cat#HPA004119, RRID:AB_1079723  

VALIDATION SUMMARY
 
 

Antibody HPA000962

  

Antibody HPA004119

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000962

  

Antibody HPA004119

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane & cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.  

Antibody dilution

 1:56   1:100  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000962

  

Antibody HPA004119

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 29 cell types		   RNA: detected in 36 tissues
Protein: detected in 45 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:750   1:350  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000962

  

Antibody HPA004119

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 97.1, 93.1   97.1, 94.1, 93.1  

Antibody dilution

 1:500   1:250  

PROTEIN ARRAY
 
 

Antibody HPA000962

  

Antibody HPA004119

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA000962

  

Antibody HPA004119

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 119   133  

Antigen sequence

 
MRIDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFKDII
NMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWNTMVLKNIAASGKFS
SDRTIKEYAQNIWNVEPSD
  
VVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQVAIQLNDTHPALAIPE
LMRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDLVEKLLPR
HLEIIYEINQKHLDRIVALFPKDVDRLRRMSLI
 

Matching transcripts

 PYGL-001 - ENSP00000216392 [100%]
PYGL-002 - ENSP00000443787 [100%]
  PYGL-001 - ENSP00000216392 [100%]
PYGL-002 - ENSP00000443787 [100%]
PYGL-003 - ENSP00000431657 [100%]
 

Other gene match

    
ANTIGEN VIEW
PYGL-001
PYGL-002
PYGL-003

RELEVANT PUBLICATIONS
 
 

Antibody HPA000962

  

Antibody HPA004119

  

1.

 Expression of glycogen phosphorylase isoforms in cultured muscle from patients with McArdle's disease carrying the p.R771PfsX33 PYGM mutation
Nogales-Gadea G et al
PLoS One 2010;5(10):
    

2.

 AMPK antagonizes hepatic glucagon-stimulated cyclic AMP signalling via phosphorylation-induced activation of cyclic nucleotide phosphodiesterase 4B
Johanns M et al
Nat Commun 2016;7:10856
Application: WB 		   
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.