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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol.
Antibody dilution
1:115
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA036070
Antibody HPA036071
Antibody CAB032952
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells and decidual cells.
Immunohistochemical staining of human testis shows strong nuclear positivity in primary spermatocytes of seminiferous ducts..
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 19 tissues Protein: detected in 53 cell types
RNA: detected in 19 tissues Protein: detected in 51 cell types
RNA: detected in 19 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:85
1:100
1:40
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036070
Antibody HPA036071
Antibody CAB032952
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416957)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416957)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
51.8
51.8
51.8, 14.5
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA036070
Antibody HPA036071
Antibody CAB032952
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
