NOP56 - Antibodies - The Human Protein Atlas

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NOP56

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA049918

Antibody CAB075757

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA049918

AMAb91013

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA049918, RRID:AB_2680952

Atlas Antibodies Cat#AMAb91013, RRID:AB_2665763

VALIDATION SUMMARY

Antibody HPA049918

Antibody CAB075757

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA049918

Antibody CAB075757

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.

Antibody dilution

1:27

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA049918

Antibody CAB075757

Standard validation

Supported

Figure description

Immunohistochemical staining of human cerebral cortex shows strong nucleolar positivity in neuronal cells.

Immunohistochemical staining of human fallopian tube shows moderate nucleolar positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 23 cell types

RNA: detected in 37 tissues
Protein: detected in 78 cell types

Retrieval

HIER pH6

Antibody dilution

1:35

1:700

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA049918

Antibody CAB075757

Formal validation: Genetic

Validated

Downregulation visible in one of two siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

Loading control

Total protein image

Antibody dilution

1:61

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

66, 31.3, 24.1

Antibody dilution

1:140

1:500

PROTEIN ARRAY

Antibody HPA049918

Antibody CAB075757

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1100

ANTIGEN INFORMATION

Antibody HPA049918

Antibody CAB075757

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

EDPSISFSKPKKKKSFSKEELMSSDLEETAGSTS

Matching transcripts

NOP56-009 - ENSP00000370589 [100%]

Other gene match

ANTIGEN VIEW

NOP56-009

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.