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Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Antibody dilution
1:63
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA067599
Antibody CAB070417
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells and moderate cytoplasmic positivity in cells in seminiferous ducts.
Expression
RNA: detected in 36 tissues Protein: detected in 14 cell types
Retrieval
HIER pH6
Antibody dilution
1:2500
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA067599
Antibody CAB070417
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
22.1
35.1, 34.8, 22.1, 18.9, 16.1
Antibody dilution
1:140
1:500
PROTEIN ARRAY
Antibody HPA067599
Antibody CAB070417
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
