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ANTIBODY INFORMATION
 
 

Antibody HPA002858

  

Antibody CAB000076

  

Antibody CAB055505

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
   Novocastra
  

Product name

 HPA002858   A0010   NCL-L-SYNAP-299  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   msAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity   Not known  

Other gene match

      

Released in version

 3   1   12  

References

      

Proper citation

 Atlas Antibodies Cat#HPA002858, RRID:AB_1080153   Dako Cat#A0010, RRID:AB_2315411    

VALIDATION SUMMARY
 
 

Antibody HPA002858

  

Antibody CAB000076

  

Antibody CAB055505

  
Immunohistochemistry

Supported   Supported  

Validated
  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002858

  

Antibody CAB000076

  

Antibody CAB055505

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuropil.   Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuropil.   Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.  

Expression

 RNA: detected in 31 tissues
Protein: detected in 34 cell types		   RNA: detected in 31 tissues
Protein: detected in 28 cell types		   RNA: detected in 31 tissues
Protein: detected in 17 cell types		  

Retrieval

 HIER pH6   HIER pH9   HIER pH6  

Antibody dilution

 1:25   1:150   1:150  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002858

  

Antibody CAB000076

  

Antibody CAB055505

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 33.8, 21.4   33.8, 21.4   33.8, 21.4  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002858

  

Antibody CAB000076

  

Antibody CAB055505

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:2000      

ANTIGEN INFORMATION
 
 

Antibody HPA002858

  

Antibody CAB000076

  

Antibody CAB055505

  

Antigen

 Recombinant protein fragment   Synthetic peptide   Synthetic peptide  

Length (aa)

 35      

Antigen sequence

 
DMDVVNQLVAGGQFRVVKEPLGFVKVLQWAAPSVL
     

Matching transcripts

 SYP-001 - ENSP00000263233 [83%]
SYP-002 - ENSP00000418387 [83%]
SYP-004 - ENSP00000418169 [83%]
     

Other gene match

      
ANTIGEN VIEW
SYP-001
SYP-002
SYP-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.