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ANTIBODY INFORMATION
 
 

Antibody HPA046852

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA046852  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 10  

References

  

Proper citation

 Atlas Antibodies Cat#HPA046852, RRID:AB_2679840  

VALIDATION SUMMARY
 
 

Antibody HPA046852

  
Immunocytochemistry

Approved  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA046852

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & cytosol.  

Antibody dilution

 1:48  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046852

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human heart muscle shows moderate nuclear positivity in myocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 68 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:200  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA046852

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 57.8  

Antibody dilution

 1:100  

PROTEIN ARRAY
 
 

Antibody HPA046852

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1950  

ANTIGEN INFORMATION
 
 

Antibody HPA046852

  

Antigen

 Recombinant protein fragment  

Length (aa)

 24  

Antigen sequence

 
DPCLIPEATQGGTYNFDPTANLQT
 

Matching transcripts

 KPNA3-001 - ENSP00000261667 [100%]
 

Other gene match

  
ANTIGEN VIEW
KPNA3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.