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ANTIBODY INFORMATION
 
 

Antibody HPA041220

  

Antibody HPA058036

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041220   HPA058036  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 14   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA041220, RRID:AB_2677367   Atlas Antibodies Cat#HPA058036, RRID:AB_2683585  

VALIDATION SUMMARY
 
 

Antibody HPA041220

  

Antibody HPA058036

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

  Approved  
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041220

  

Antibody HPA058036

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA058036.		  
Validated

Antibody staining overlaps with antibody HPA041220.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.   Immunofluorescent staining of human cell line MCF7 shows localization to nuclear bodies.  

Antibody dilution

 1:189   1:149  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041220

  

Antibody HPA058036

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human cerebral cortex shows moderate nucleolar positivity in neuronal cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 34 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:375  

Literature conformity

   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041220

  

Antibody HPA058036

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 60.4, 55.1   60.4  

Antibody dilution

 1:250   1:490  

PROTEIN ARRAY
 
 

Antibody HPA041220

  

Antibody HPA058036

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:5950  

ANTIGEN INFORMATION
 
 

Antibody HPA041220

  

Antibody HPA058036

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 107   95  

Antigen sequence

 
RGRSHGARSVGRSAHIQASGHLEEQTPRTLLKNILLTAPESSILMPESVV
KPVPAPQAVQPSRQESSCGSLELQLPELEPPTTLAPGLLAPGRRKQRLRL
SVFQQGV
  
PLHDGVEEAEKKMEEEGVSVSEMEATGAQGPSRVEEAEGHTEVTEAEGSQ
GTAEADGPGASSGDEDASGRAASPESASSTPESLQARRHHQFLEP
 

Matching transcripts

 CENPT-001 - ENSP00000457810 [100%]
CENPT-008 - ENSP00000457294 [100%]
CENPT-201 - ENSP00000400140 [100%]
  CENPT-001 - ENSP00000457810 [100%]
CENPT-201 - ENSP00000400140 [100%]
 

Other gene match

    
ANTIGEN VIEW
CENPT-001
CENPT-008
CENPT-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.