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ANTIBODY INFORMATION
 
 

Antibody HPA043313

  

Antibody HPA063634

  

Antibody CAB034422

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA043313   HPA063634   sc-133147  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   14   7  

References

      

Proper citation

 Atlas Antibodies Cat#HPA043313, RRID:AB_2678421   Atlas Antibodies Cat#HPA063634, RRID:AB_2685067   Santa Cruz Biotechnology Cat#sc-133147, RRID:AB_2110748  

VALIDATION SUMMARY
 
 

Antibody HPA043313

  

Antibody HPA063634

  

Antibody CAB034422

  
Immunocytochemistry


Validated
  

Validated
   Supported  
Immunohistochemistry

    Approved  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043313

  

Antibody HPA063634

  

Antibody CAB034422

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA063634.		  
Validated

Antibody staining overlaps with antibody HPA043313.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line RH-30 shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.  

Antibody dilution

 1:103   1:105   1:200  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043313

  

Antibody HPA063634

  

Antibody CAB034422

  

Standard validation

     Approved  

     

Figure description

     Immunohistochemical staining of human stomach shows strong luminal membrane and cytoplasmic positivity in glandular cells.  

Expression

     RNA: detected in 37 tissues
Protein: detected in 59 cell types		  

Retrieval

     HIER pH6  

Antibody dilution

     1:1000  

Literature conformity

     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043313

  

Antibody HPA063634

  

Antibody CAB034422

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 67.1   67.1   67.1, 24.8, 14, 13.4, 12.1  

Antibody dilution

 1:250   1:240   1:500  

PROTEIN ARRAY
 
 

Antibody HPA043313

  

Antibody HPA063634

  

Antibody CAB034422

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:6000   1:4250    

ANTIGEN INFORMATION
 
 

Antibody HPA043313

  

Antibody HPA063634

  

Antibody CAB034422

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 85   80    

Antigen sequence

 
ANDLLTQGVLLYKQVMEGRVTFGNRVTSSLGDIPVSRVFQNPAGCMKTCP
LIDLEVDNGPAQMGTVVPSLLHQDLAALGISDAPV
  
NCCEEKRNPSSSTLPGGGVQNPSADRNLLDLLSPQPAPCPLNYVSQKSVP
KEVPPGTKSSPGWSWEAGPLAPSPSSQNTP
   

Matching transcripts

 GGA2-001 - ENSP00000311962 [100%]
  GGA2-001 - ENSP00000311962 [99%]
   

Other gene match

      
ANTIGEN VIEW
GGA2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.