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STX4

ANTIBODY INFORMATION
 
 

Antibody HPA001330

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001330  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 3  

References

 2  

Proper citation

 Atlas Antibodies Cat#HPA001330, RRID:AB_1080167  

VALIDATION SUMMARY
 
 

Antibody HPA001330

  
Immunocytochemistry

Approved  
Immunohistochemistry

Approved  
Western blot


Validated
  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001330

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.  

Antibody dilution

 1:143  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001330

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human skin shows membranous positivity in epidermal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 61 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:350  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001330

  

Formal validation: Genetic
Validated

Downregulation visible in one of two siRNA lanes		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		  

Target mass (kDa)

 34.2  

Loading control

 Total protein image  

Antibody dilution

 1:314  
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 34.2  

Antibody dilution

 1:500  

PROTEIN ARRAY
 
 

Antibody HPA001330

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA001330

  

Antigen

 Recombinant protein fragment  

Length (aa)

 147  

Antigen sequence

 
MRDRTHELRQGDDSSDEEDKERVALVVHPGTARLGSPDEEFFHKVRTIRQ
TIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQ
LKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSE
 

Matching transcripts

 STX4-001 - ENSP00000317714 [100%]
 

Other gene match

  
ANTIGEN VIEW
STX4-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA001330

  

1.

 Protein expression of PKCZ (Protein Kinase C Zeta), Munc18c, and Syntaxin-4 in the insulin pathway in endometria of patients with polycystic ovary syndrome (PCOS)
Rivero R et al
Reprod Biol Endocrinol 2012;10:17
  

2.

 Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome
Campbell-Valois FX et al
Mol Cell Proteomics 2012;11(7):M111.016378
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.