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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity, with a granular pattern, in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in granular pattern in tubular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 41 cell types
RNA: detected in 37 tissues Protein: detected in 36 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:400
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003524
Antibody CAB000458
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
37.4, 36.3
37.4, 36.3, 14.7
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA003524
Antibody CAB000458
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
