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TJP1

ANTIBODY INFORMATION
 
 

Antibody HPA001636

  

Antibody HPA001637

  

Antibody CAB010822

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA001636   HPA001637   1712.00.02  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 2   2   4  

References

 2   2    

Proper citation

 Atlas Antibodies Cat#HPA001636, RRID:AB_1080762   Atlas Antibodies Cat#HPA001637, RRID:AB_1080760    

VALIDATION SUMMARY
 
 

Antibody HPA001636

  

Antibody HPA001637

  

Antibody CAB010822

  
Immunocytochemistry


Validated
  

Validated
   Supported  
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  

Validated
  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001636

  

Antibody HPA001637

  

Antibody CAB010822

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA001637.		  
Validated

Antibody staining overlaps with antibody HPA001636.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & cell junctions.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & cell junctions.   Immunofluorescent staining of human cell line U-251 MG shows localization to cell junctions.  

Antibody dilution

 1:34   1:26   1:100  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001636

  

Antibody HPA001637

  

Antibody CAB010822

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

Cell line01,0002,0003,0004,0005,0006,000Protein expression
Cell line0102030405060RNA expression
 		 
N/AN/ACell line02,0004,0006,0008,000Protein expression
Cell line0102030405060RNA expression
 		 
Cell line01,0002,0003,0004,0005,0006,0007,000Protein expression
Cell line0102030405060RNA expression
 		 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

N/AN/AN/AN/AN/ATissue cell typeNotdetectedLowMediumHighProtein expression
 		 
N/AN/ATissue cell typeNotdetectedLowMediumHighProtein expression
 		   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA001637 across 70 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA001636 across 70 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human kidney shows distinct membranous positivity in cells in glomeruli.   Immunohistochemical staining of human kidney shows distinct membranous and cytoplasmic positivity in cells of glomeruli.   Immunohistochemical staining of human kidney shows distinct membranous positivity in glomerular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 56 cell types		   RNA: detected in 36 tissues
Protein: detected in 72 cell types		   RNA: detected in 36 tissues
Protein: detected in 47 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:35   1:400  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001636

  

Antibody HPA001637

  

Antibody CAB010822

  

Standard validation

 Supported

Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418804)		      

Target mass (kDa)

 197.5, 195.5, 189.7, 187.8, 187   197.5, 195.5, 189.7, 187.8, 187, 42.5   197.5, 195.5, 189.7, 187.8, 187, 42.5, 25.9, 24.6  

Antibody dilution

 1:125   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001636

  

Antibody HPA001637

  

Antibody CAB010822

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA001636

  

Antibody HPA001637

  

Antibody CAB010822

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 133   126    

Antigen sequence

 
RKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVS
EGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGG
AYTDQELDETLNDEVGTPPESAITRSSEPVRED
  
ASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEG
DVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSD
SIHSANASERDDISEIQSLASDHSGR
  

											 
																				

																		

										
											
Matching transcripts

												
																					TJP1-001 - ENSP00000281537 [100%] 
TJP1-002 - ENSP00000382890 [100%] 
TJP1-008 - ENSP00000441202 [100%] 
TJP1-009 - ENSP00000348416 [100%] 
TJP1-201 - ENSP00000483470 [100%] 

											 
																						TJP1-001 - ENSP00000281537 [100%] 
TJP1-002 - ENSP00000382890 [100%] 
TJP1-003 - ENSP00000452729 [100%] 
TJP1-008 - ENSP00000441202 [100%] 
TJP1-009 - ENSP00000348416 [100%] 
TJP1-201 - ENSP00000483470 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TJP1-001
TJP1-002
TJP1-003
TJP1-008
TJP1-009
TJP1-201
								

							

						

							
									

			

				
	

	

		
			
				
	

													
															
				
											

									
																				
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001636

												
										 
																				
											
											
											
Antibody HPA001637

												
										 
																				
											
											
											
Antibody CAB010822

												
										 
																		

																	

										
											
1.

												
																						Expansive generation of functional airway epithelium from human embryonic stem cells

	McIntyre BA et al

	Stem Cells Transl Med 2014;3(1):7-17

			Application: IHC				
											 
																							Sequential development of intercellular junctions in bioengineered human corneas

	González-Andrades M et al

	J Tissue Eng Regen Med 2009;3(6):442-9

			
											 
																						
											 
																				

																		

										
											
2.

												
																						A recellularized human colon model identifies cancer driver genes

	Chen HJ et al

	Nat Biotechnol 2016;34(8):845-51

			Application: ICC-IF				
											 
																							Cigarette smoke facilitates allergen penetration across respiratory epithelium

	Gangl K et al

	Allergy 2009;64(3):398-405

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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