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Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.
Antibody dilution
1:99
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA006880
Antibody HPA053715
Antibody CAB033274
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human hippocampus shows cytoplasmic positivity in neurons.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.
Expression
RNA: detected in 12 tissues Protein: detected in 9 cell types
RNA: detected in 12 tissues Protein: detected in 11 cell types
RNA: detected in 12 tissues Protein: detected in 10 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1500
1:1300
1:450
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Standard validation
Approved
Figure description
Synaptic positivity observed in selected regions of the brain, most abundant in circumventricular organs and hypothalamus regions.
WESTERN BLOT
Antibody HPA006880
Antibody HPA053715
Antibody CAB033274
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402232)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
53
53, 26.9
53, 26.9, 6.5
Antibody dilution
1:250
1:490
1:500
PROTEIN ARRAY
Antibody HPA006880
Antibody HPA053715
Antibody CAB033274
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
