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CCNE1

ANTIBODY INFORMATION
 
 

Antibody HPA018169

  

Antibody CAB000308

  

Antibody CAB016682

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Pharmingen
   AbFrontier
  

Product name

 HPA018169   551160   LF-PA0168  

Host species

 Rabbit   Mouse   Rabbit  

Clonality

 pAb   mAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G  

Other gene match

      

Released in version

 5   1   4  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA018169, RRID:AB_1847384     ABFrontier Cat#LF-PA0168, RRID:AB_1614564  

VALIDATION SUMMARY
 
 

Antibody HPA018169

  

Antibody CAB000308

  

Antibody CAB016682

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Supported   Supported  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA018169

  

Antibody CAB000308

  

Antibody CAB016682

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.    

Antibody dilution

 1:172   1:50    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA018169

  

Antibody CAB000308

  

Antibody CAB016682

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts.   Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows cytoplasmic and nuclear positivity in trophoblastic cells.  

Expression

 RNA: detected in 31 tissues
Protein: detected in 61 cell types		   RNA: detected in 31 tissues
Protein: detected in 44 cell types		   RNA: detected in 31 tissues
Protein: detected in 59 cell types		  

Retrieval

 HIER pH6   HIER pH9   HIER pH6  

Antibody dilution

 1:85   1:200   1:100  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA018169

  

Antibody CAB000308

  

Antibody CAB016682

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 47.1, 45.1, 39.8, 25.2   47.1, 45.1, 39.8, 32.4, 25.2   47.1, 45.1, 39.8, 32.4, 25.2  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA018169

  

Antibody CAB000308

  

Antibody CAB016682

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA018169

  

Antibody CAB000308

  

Antibody CAB016682

  

Antigen

 Recombinant protein fragment   Not known   Recombinant protein  

Length (aa)

 67      

Antigen sequence

 
RDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPW
DNNAVCADPCSLIPTPD
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CCNE1-001 - ENSP00000262643 [100%] 
CCNE1-002 - ENSP00000410179 [96%] 
CCNE1-004 - ENSP00000459024 [96%] 
CCNE1-201 - ENSP00000350625 [96%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CCNE1-001
CCNE1-002
CCNE1-004
CCNE1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA018169

												
										 
																				
											
											
											
Antibody CAB000308

												
										 
																				
											
											
											
Antibody CAB016682

												
										 
																		

																	

										
											
1.

												
																						The 19q12 bladder cancer GWAS signal: association with cyclin E function and aggressive disease

	Fu YP et al

	Cancer Res 2014;74(20):5808-18

			Application: IHC				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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