Fields »
ABOUT
HELP
BLOG
EBI3

ANTIBODY INFORMATION
 
 

Antibody HPA046635

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA046635  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 12  

References

  

Proper citation

 Atlas Antibodies Cat#HPA046635, RRID:AB_2679729  

VALIDATION SUMMARY
 
 

Antibody HPA046635

  
Immunohistochemistry


Validated
  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046635

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.  

Expression

 RNA: detected in 27 tissues
Protein: detected in 18 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA046635

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 25.4  

Antibody dilution

 1:560  

PROTEIN ARRAY
 
 

Antibody HPA046635

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:4450  

ANTIGEN INFORMATION
 
 

Antibody HPA046635

  

Antigen

 Recombinant protein fragment  

Length (aa)

 136  

Antigen sequence

 
SPVSFIATYRLGMAARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVT
AVHPWGSSSSFVPFITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPF
PEIFSLKYWIRYKRQGAARFHRVGPIEATSFILRAV
 

Matching transcripts

 EBI3-001 - ENSP00000221847 [100%]
 

Other gene match

  
ANTIGEN VIEW
EBI3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.