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Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Antibody dilution
1:10
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA019735
Standard validation
Approved
Figure description
Immunohistochemical staining of human tonsil shows strong positivity in cells outsede the reaction centra while cells in the reaction centra were weaker stained.
Expression
RNA: detected in 37 tissues Protein: detected in 51 cell types
Retrieval
HIER pH6
Antibody dilution
1:425
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019735
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
12.9
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA019735
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
