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ANTIBODY INFORMATION
 
 

Antibody HPA019049

  

Antibody HPA019051

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA019049   HPA019051  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   5  

References

    

Proper citation

 Atlas Antibodies Cat#HPA019049, RRID:AB_1846163   Atlas Antibodies Cat#HPA019051, RRID:AB_1846164  

VALIDATION SUMMARY
 
 

Antibody HPA019049

  

Antibody HPA019051

  
Immunocytochemistry

  Supported  
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  
Western blot

Uncertain   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019049

  

Antibody HPA019051

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.  

Antibody dilution

   1:88  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019049

  

Antibody HPA019051

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA019051 across 45 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA019049 across 45 cell lines.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.   Immunohistochemical staining of human fallopian tube shows distinct cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 28 cell types		   RNA: detected in 37 tissues
Protein: detected in 55 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:275  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019049

  

Antibody HPA019051

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 53.3   53.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA019049

  

Antibody HPA019051

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA019049

  

Antibody HPA019051

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 112   84  

Antigen sequence

 
QPVSAPPSPRDISMEIDSPENMMRHIRFLKNEVERLKKQLRAAQLQHSEK
MAQYLEEERHMREENLRLQRKLQREMERREALCRQLSESESSLEMDDERY
FNEMSAQGLRPR
  
FKKIQALQKEKETLAVNYEKEEEFLTNELSRKLMQLQHEKAELEQHLEQE
QEFQVNKLMKKIKKLENDTISKQLTLEQLRREKI
 

Matching transcripts

 CCDC6-001 - ENSP00000263102 [100%]
  CCDC6-001 - ENSP00000263102 [100%]
 

Other gene match

    
ANTIGEN VIEW
CCDC6-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.