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FTSJ3

ANTIBODY INFORMATION
 
 

Antibody HPA055544

  

Antibody HPA062628

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA055544   HPA062628  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA055544, RRID:AB_2682843   Atlas Antibodies Cat#HPA062628, RRID:AB_2684813  

VALIDATION SUMMARY
 
 

Antibody HPA055544

  

Antibody HPA062628

  
Immunocytochemistry

Supported  

Validated
  
Immunohistochemistry

Supported    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA055544

  

Antibody HPA062628

  

Formal validation: Genetic

  
Validated

Significant downregulation > 25% by both siRNA:s.		  

 
 

Figure description

   Nuclear region of segmented cells in 10x-images  

Antibody dilution

   1:154  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.  

Antibody dilution

 1:200   1:154  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA055544

  

Antibody HPA062628

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human cerebellum shows strong nucleolar positivity in Purkinje cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 33 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:1000    

Literature conformity

 Consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA055544

  

Antibody HPA062628

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 96.6   96.6  

Antibody dilution

 1:1020   1:510  

PROTEIN ARRAY
 
 

Antibody HPA055544

  

Antibody HPA062628

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:8150   1:6150  

ANTIGEN INFORMATION
 
 

Antibody HPA055544

  

Antibody HPA062628

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 115   64  

Antigen sequence

 
FVVCQGFLAPDKVDSKFFDPKFAFKEVEVQAKTVTELVTKKKPKAEGYAE
GDLTLYHRTSVTDFLRAANPVDFLSKASEIMVDDEELAQHPATTEDIRVC
CQDIRVLGRKELRSL
  
KAVLQEEQANLWFSKGSFAGIEDDADEALEISQAQLLFENRRKGRQQQQK
QQLPQTPPSCLKTE
 

Matching transcripts

 FTSJ3-001 - ENSP00000396673 [100%]
  FTSJ3-001 - ENSP00000396673 [100%]
 

Other gene match

    
ANTIGEN VIEW
FTSJ3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.