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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.
Antibody dilution
1:20
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA021408
Antibody HPA022869
Antibody HPA023714
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in bone marrow poietic cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of the seminiferus ducts.
Expression
RNA: detected in 15 tissues Protein: detected in 38 cell types
RNA: detected in 15 tissues Protein: detected in 54 cell types
RNA: detected in 15 tissues Protein: detected in 46 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:3000
1:200
1:400
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA021408
Antibody HPA022869
Antibody HPA023714
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410824)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
65.7, 58.8, 55.1, 45.5, 21.6
65.7, 58.8, 55.1, 45.5
65.7, 58.8, 55.1, 45.5, 15.5
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA021408
Antibody HPA022869
Antibody HPA023714
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:3000
1:500
ANTIGEN INFORMATION
Antibody HPA021408
Antibody HPA022869
Antibody HPA023714
Antigen
Recombinant protein fragment
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
101
102
98
Antigen sequence
LKKVSTKRPPPAMDDLDFTTTVVSCCPAELQTEGSNGKKEVLSGFQVVLE
DTVLFPEGGGQPDDRGTINDISVLRVTRRGEQADHFTQTPLDPGSQVLVR
V
GGVVILHRKEGDSEFMNIIANEIGSEETLLFLTVGDEKGGGLFLLAGPPA
SVETLGPRVAEVLEGKGAGKKGRFQGKATKMSRRMEAQALLQDYISTQSA
KE
