We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Fields »
 

Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
ABOUT
HELP
BLOG
  • THE HUMAN PROTEOME
  • THE HUMAN TISSUES
  • THE HUMAN CELL
  • HUMAN PATHOLOGY
  • PROTEIN CLASSES
  • PROTEIN EVIDENCE
    • NEWS
    • BLOG
    • EVENTS
    • PRESS ROOM
    • LEARN
    • DICTIONARY
    • METHODS
    • CELL LINES
  • THE PROJECT
  • INTRODUCTION
  • ORGANIZATION
  • PUBLICATIONS
  • PUBLICATION DATA
  • ANTIBODY SUBMISSION
  • ANTIBODY AVAILABILITY
  • LINKS
  • CONTACT
  • TECHNICAL DATA
  • ANTIBODY VALIDATION
  • ASSAYS & ANNOTATION
  • DISCLAIMER
  • DOWNLOADABLE DATA
  • HELP & FAQ
  • LICENCE & CITATION
  • PRIVACY STATEMENT
  • RELEASE HISTORY
SLC9A3R1
  • premium

    TISSUE

  • CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

SLC9A3R1

Gene description

Solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1

Protein class

Disease related genes
Plasma proteins
Potential drug targets
Predicted intracellular proteins
Transporters

Predicted localization

Intracellular

Number of transcripts

3
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and membranous expression in most tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data.

Reliability score

Supported based on 3 antibodies.
HPA009672 , HPA027247 , CAB001962
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in liver cancer (unfavourable).
Liver cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Endometrial cancer

STAINING SUMMARY

HPA009672
HPA027247
CAB001962

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Distinct membranous and cytoplasmic staining was observed in fractions of cases in most adenocarcinomas. Cancer cells in general were weakly stained or negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate to strong cytoplasmic positivity was observed in breast cancers while several cases of endometrial, ovaria renal and embryonal carcinomas of testis displayed strong luminal membrane staining. Nuclear positivity was occasionally observed in liver, lung, breast and renal cancers. Remaining cancer tissues were mainly negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancer tissues showed strong cytoplasmic positivity with additional membranous immunoreactivity. Hepatocellular cancer were mainly negative.

GENE INFORMATION

Gene name

SLC9A3R1 (HGNC Symbol)

Synonyms

EBP50, NHERF

Description

Solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1 (HGNC Symbol)

Entrez gene summary

This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.[provided by RefSeq, Sep 2009]

Chromosome

17

Cytoband

q25.1

Chromosome location (bp)

74748652 - 74769353

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000109062 (version 83.38)

Entrez gene

9368

UniProt

O14745 (UniProt - Evidence at protein level)

neXtProt

NX_O14745

Antibodypedia

SLC9A3R1 antibodies

PROTEIN BROWSER

SLC9A3R1-001
SLC9A3R1-002
SLC9A3R1-006

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

SLC9A3R1-001
ENSP00000262613
ENST00000262613
O14745 [Direct mapping]
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
Show all
Transporters
   Accessory Factors Involved in Transport
Predicted intracellular proteins
Plasma proteins
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001726 [ruffle]
GO:0003096 [renal sodium ion transport]
GO:0005102 [receptor binding]
GO:0005515 [protein binding]
GO:0005737 [cytoplasm]
GO:0005813 [centrosome]
GO:0005886 [plasma membrane]
GO:0005902 [microvillus]
GO:0006461 [protein complex assembly]
GO:0007191 [adenylate cyclase-activating dopamine receptor signaling pathway]
GO:0007605 [sensory perception of sound]
GO:0008013 [beta-catenin binding]
GO:0008285 [negative regulation of cell proliferation]
GO:0008360 [regulation of cell shape]
GO:0008361 [regulation of cell size]
GO:0010642 [negative regulation of platelet-derived growth factor receptor signaling pathway]
GO:0010766 [negative regulation of sodium ion transport]
GO:0012505 [endomembrane system]
GO:0014067 [negative regulation of phosphatidylinositol 3-kinase signaling]
GO:0015629 [actin cytoskeleton]
GO:0016020 [membrane]
GO:0016055 [Wnt signaling pathway]
GO:0016324 [apical plasma membrane]
GO:0017081 [chloride channel regulator activity]
GO:0019902 [phosphatase binding]
GO:0019904 [protein domain specific binding]
GO:0022612 [gland morphogenesis]
GO:0030033 [microvillus assembly]
GO:0030036 [actin cytoskeleton organization]
GO:0030165 [PDZ domain binding]
GO:0030175 [filopodium]
GO:0030336 [negative regulation of cell migration]
GO:0030643 [cellular phosphate ion homeostasis]
GO:0031526 [brush border membrane]
GO:0031528 [microvillus membrane]
GO:0031698 [beta-2 adrenergic receptor binding]
GO:0031982 [vesicle]
GO:0032403 [protein complex binding]
GO:0032415 [regulation of sodium:proton antiporter activity]
GO:0032416 [negative regulation of sodium:proton antiporter activity]
GO:0032420 [stereocilium]
GO:0032426 [stereocilium bundle tip]
GO:0032782 [bile acid secretion]
GO:0032947 [protein complex scaffold]
GO:0034613 [cellular protein localization]
GO:0034635 [glutathione transport]
GO:0035414 [negative regulation of catenin import into nucleus]
GO:0040023 [establishment of nucleus localization]
GO:0043231 [intracellular membrane-bounded organelle]
GO:0043621 [protein self-association]
GO:0044062 [regulation of excretion]
GO:0045121 [membrane raft]
GO:0045177 [apical part of cell]
GO:0045198 [establishment of epithelial cell apical/basal polarity]
GO:0045859 [regulation of protein kinase activity]
GO:0045930 [negative regulation of mitotic cell cycle]
GO:0048471 [perinuclear region of cytoplasm]
GO:0050780 [dopamine receptor binding]
GO:0051683 [establishment of Golgi localization]
GO:0051898 [negative regulation of protein kinase B signaling]
GO:0060088 [auditory receptor cell stereocilium organization]
GO:0060158 [phospholipase C-activating dopamine receptor signaling pathway]
GO:0070062 [extracellular exosome]
GO:0070293 [renal absorption]
GO:0070373 [negative regulation of ERK1 and ERK2 cascade]
GO:0070851 [growth factor receptor binding]
GO:0071944 [cell periphery]
GO:0090002 [establishment of protein localization to plasma membrane]
GO:0097225 [sperm midpiece]
GO:0097291 [renal phosphate ion absorption]
GO:2001244 [positive regulation of intrinsic apoptotic signaling pathway]
Show all
358 aa
38.9 kDa
No 0
SLC9A3R1-002
ENSP00000464982
ENST00000413388
O14745 [Direct mapping]
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
Show all
Transporters
   Accessory Factors Involved in Transport
Predicted intracellular proteins
Plasma proteins
Disease related genes
Potential drug targets
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001726 [ruffle]
GO:0005102 [receptor binding]
GO:0005515 [protein binding]
GO:0005737 [cytoplasm]
GO:0005813 [centrosome]
GO:0005886 [plasma membrane]
GO:0005902 [microvillus]
GO:0006461 [protein complex assembly]
GO:0008013 [beta-catenin binding]
GO:0008285 [negative regulation of cell proliferation]
GO:0008360 [regulation of cell shape]
GO:0008361 [regulation of cell size]
GO:0010642 [negative regulation of platelet-derived growth factor receptor signaling pathway]
GO:0012505 [endomembrane system]
GO:0014067 [negative regulation of phosphatidylinositol 3-kinase signaling]
GO:0015629 [actin cytoskeleton]
GO:0016020 [membrane]
GO:0016055 [Wnt signaling pathway]
GO:0016324 [apical plasma membrane]
GO:0017081 [chloride channel regulator activity]
GO:0019902 [phosphatase binding]
GO:0022612 [gland morphogenesis]
GO:0030033 [microvillus assembly]
GO:0030165 [PDZ domain binding]
GO:0030175 [filopodium]
GO:0031528 [microvillus membrane]
GO:0031698 [beta-2 adrenergic receptor binding]
GO:0031982 [vesicle]
GO:0032415 [regulation of sodium:proton antiporter activity]
GO:0032782 [bile acid secretion]
GO:0034635 [glutathione transport]
GO:0035414 [negative regulation of catenin import into nucleus]
GO:0040023 [establishment of nucleus localization]
GO:0043231 [intracellular membrane-bounded organelle]
GO:0043621 [protein self-association]
GO:0045198 [establishment of epithelial cell apical/basal polarity]
GO:0045859 [regulation of protein kinase activity]
GO:0045930 [negative regulation of mitotic cell cycle]
GO:0048471 [perinuclear region of cytoplasm]
GO:0051683 [establishment of Golgi localization]
GO:0051898 [negative regulation of protein kinase B signaling]
GO:0070062 [extracellular exosome]
GO:0070293 [renal absorption]
GO:0070373 [negative regulation of ERK1 and ERK2 cascade]
GO:0070851 [growth factor receptor binding]
GO:0071944 [cell periphery]
GO:0090002 [establishment of protein localization to plasma membrane]
GO:0097225 [sperm midpiece]
GO:0097291 [renal phosphate ion absorption]
GO:2001244 [positive regulation of intrinsic apoptotic signaling pathway]
Show all
202 aa
22 kDa
No 0
SLC9A3R1-006
ENSP00000464321
ENST00000583369
J3QRP6 [Direct mapping]
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
GO:0005886 [plasma membrane]
GO:0032947 [protein complex scaffold]
GO:0043231 [intracellular membrane-bounded organelle]
Show all
215 aa
22.9 kDa
No 0

Contact

  • BLOG
  • PRESS ROOM
  • contact@proteinatlas.org

The Project

  • INTRODUCTION
  • ORGANIZATION
  • PUBLICATIONS
  • DOWNLOADABLE DATA

The Human Protein Atlas

  • ANTIBODY AVAILABILITY
  • LICENCE & CITATION
  • HELP & FAQ
The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.