Fields »
ABOUT
HELP
BLOG
UNC93B1

ANTIBODY INFORMATION
 
 

Antibody HPA038716

  

Antibody HPA038717

  

Antibody HPA042803

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA038716   HPA038717   HPA042803  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 14   7   10  

References

      

Proper citation

 Atlas Antibodies Cat#HPA038716, RRID:AB_2676169   Atlas Antibodies Cat#HPA038717, RRID:AB_10674836   Atlas Antibodies Cat#HPA042803, RRID:AB_2678176  

VALIDATION SUMMARY
 
 

Antibody HPA038716

  

Antibody HPA038717

  

Antibody HPA042803

  
Immunocytochemistry

Approved      
Immunohistochemistry

  Approved   Approved  
Western blot

Supported   Uncertain   Supported  
Protein array

Approved   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038716

  

Antibody HPA038717

  

Antibody HPA042803

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.      

Antibody dilution

 1:132      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038716

  

Antibody HPA038717

  

Antibody HPA042803

  

Standard validation

   Approved   Approved  

     

Figure description

   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.  

Expression

   RNA: detected in 35 tissues
Protein: detected in 65 cell types		   RNA: detected in 35 tissues
Protein: detected in 69 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:20   1:50  

Literature conformity

   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038716

  

Antibody HPA038717

  

Antibody HPA042803

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 66.6, 21   66.6   66.6  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA038716

  

Antibody HPA038717

  

Antibody HPA042803

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:3000   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA038716

  

Antibody HPA038717

  

Antibody HPA042803

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 36   41   25  

Antigen sequence

 
NYITRMAQKYHEYSHYKEQDGQGMKQRPPRGSHAPY
  
NHYLYDLNHTLYNVQSCGTNSHGILSGFNKTVLRTLPRSGN
  
LNKTGLSTLLGILYEDKERQDFIFT
 

Matching transcripts

 UNC93B1-001 - ENSP00000227471 [100%]
UNC93B1-002 - ENSP00000437195 [100%]
  UNC93B1-001 - ENSP00000227471 [100%]
  UNC93B1-001 - ENSP00000227471 [100%]
 

Other gene match

      
ANTIGEN VIEW
UNC93B1-001
UNC93B1-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.