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PRPF19

ANTIBODY INFORMATION
 
 

Antibody HPA038051

  

Antibody HPA059070

  

Antibody CAB012448

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abcam plc
  

Product name

 HPA038051   HPA059070   ab27692  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 12   13   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA038051, RRID:AB_2675807   Atlas Antibodies Cat#HPA059070, RRID:AB_2683896   Abcam Cat#ab27692, RRID:AB_2170868  

VALIDATION SUMMARY
 
 

Antibody HPA038051

  

Antibody HPA059070

  

Antibody CAB012448

  
Immunocytochemistry


Validated

Validated
  

Validated

Validated
   Supported  
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported  

Validated
   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038051

  

Antibody HPA059070

  

Antibody CAB012448

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		  
Validated

Significant downregulation > 25% by both siRNA:s.		    

 
   

Figure description

 Nuclear region of segmented cells in 10x-images   Nuclear region of segmented cells in 10x-images    

Antibody dilution

 1:38   1:58    
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA059070.		  
Validated

Antibody staining overlaps with antibody HPA038051.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.   Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.   Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.  

Antibody dilution

 1:38   1:58   1:25  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038051

  

Antibody HPA059070

  

Antibody CAB012448

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA059070 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA038051 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human kidney shows strong nuclear positivity.   Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in renal tubules and cells in glomeruli.   Immunohistochemical staining of human kidney shows strong nuclear positivity.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 78 cell types		   RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:250   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038051

  

Antibody HPA059070

  

Antibody CAB012448

  

Formal validation: Genetic

  
Validated

Downregulation visible in both siRNA lanes		    

     

Figure description

   Lane 1: Marker [kDa] 250, 150, 100, 75, 50, 37, 25, 20, 15, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

   55.2    

Loading control

   Total protein image    

Antibody dilution

   1:130    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 55.2   55.2   55.2, 27.7, 13.2, 11.4, 5.3  

Antibody dilution

 1:250   1:190   1:500  

PROTEIN ARRAY
 
 

Antibody HPA038051

  

Antibody HPA059070

  

Antibody CAB012448

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:2350    

ANTIGEN INFORMATION
 
 

Antibody HPA038051

  

Antibody HPA059070

  

Antibody CAB012448

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 82   95    

Antigen sequence

 
VPNASCVQVVRAHESAVTGLSLHATGDYLLSSSDDQYWAFSDIQTGRVLT
KVTDETSGCSLTCAQFHPDGLIFGTGTMDSQI
  
LQDKATVLTTERKKRGKTVPEELVKPEELSKYRQVASHVGLHSASIPGIL
ALDLCPSDTNKILTGGADKNVVVFDKSSEQILATLKGHTKKVTSV
  

											 
																				

																		

										
											
Matching transcripts

												
																					PRPF19-001 - ENSP00000227524 [100%] 

											 
																						PRPF19-001 - ENSP00000227524 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PRPF19-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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