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PRPF19

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GENERAL INFORMATION

Gene name

 PRPF19

Gene description

 Pre-mRNA processing factor 19

Protein class

 Plasma proteins
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 5
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HUMAN PROTEIN ATLAS INFORMATION

Summary

 Mainly localized to the nuclear speckles.

RNA cell category

 Expressed in all

Protein evidence

 Evidence at protein level

Main location

 Localized to the Nuclear speckles (validated)

siRNA

 The location(s) are Validated based on siRNA mediated gene silencing.

Mouse

 Localized to the Nuclear speckles in mouse cells.

DATA RELIABILITY

Reliability score

 Validated based on 3 antibodies.
CAB012448 , HPA038051 , HPA059070
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ASSAYS
RNA expression
Human cells
siRNA
Mouse cells

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Mainly localized to the nuclear speckles.

Main location

 Nuclear speckles (validated)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
CAB012448 A-431 Nuclear speckles
Plasma membrane
CAB012448 U-2 OS Nuclear speckles
CAB012448 U-251 MG Nuclear speckles
HPA038051 A-431 Nuclear speckles
HPA038051 U-2 OS Nuclear speckles
HPA038051 U-251 MG Nuclear speckles
HPA059070 A-431 Nuclear speckles
HPA059070 MCF7 Nuclear speckles
HPA059070 U-2 OS Nuclear speckles

siRNA VALIDATION

Antibody

 HPA038051

siRNA validation

 Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

 Nuclear region of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

 30%31%

p-value

 < 0.01< 0.01
 

Antibody

 HPA059070

siRNA validation

 Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

 Nuclear region of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

 22%19%

p-value

 < 0.01< 0.01
 

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail 10x

Thumbnail 40x

Antibody

Sample

Cell line
HPA038051 siRNA 1 U-2 OS
HPA038051 siRNA 2 U-2 OS
HPA038051 scrambled U-2 OS
HPA059070 siRNA 1 U-2 OS
HPA059070 siRNA 2 U-2 OS
HPA059070 scrambled U-2 OS

MOUSE CELLS

Main location

 Nuclear speckles

Mouse gene

 ENSMUSG00000024735 (version 83)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location
HPA038051 NIH 3T3 Nuclear speckles

GENE INFORMATION

Gene name

 PRPF19 (HGNC Symbol)

Synonyms

 hPSO4, NMP200, PRP19, PSO4, UBOX4

Description

 Pre-mRNA processing factor 19 (HGNC Symbol)

Entrez gene summary

 PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]).[supplied by OMIM, Sep 2008]

Chromosome

 11

Cytoband

 q12.2

Chromosome location (bp)

 60890730 - 60906588

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000110107 (version 83.38)

Entrez gene

 27339

UniProt

 Q9UMS4 (UniProt - Evidence at protein level)

neXtProt

 NX_Q9UMS4

Antibodypedia

 PRPF19 antibodies

PROTEIN BROWSER
PRPF19-001
PRPF19-002
PRPF19-003
PRPF19-006
PRPF19-007

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
PRPF19-001
ENSP00000227524
ENST00000227524
Q9UMS4 [Direct mapping]
Pre-mRNA-processing factor 19
Show all
Predicted intracellular proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000209 [protein polyubiquitination]
GO:0000244 [spliceosomal tri-snRNP complex assembly]
GO:0000245 [spliceosomal complex assembly]
GO:0000349 [generation of catalytic spliceosome for first transesterification step]
GO:0000398 [mRNA splicing, via spliceosome]
GO:0000974 [Prp19 complex]
GO:0001833 [inner cell mass cell proliferation]
GO:0004842 [ubiquitin-protein transferase activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005662 [DNA replication factor A complex]
GO:0005737 [cytoplasm]
GO:0005811 [lipid particle]
GO:0005819 [spindle]
GO:0006281 [DNA repair]
GO:0006283 [transcription-coupled nucleotide-excision repair]
GO:0006289 [nucleotide-excision repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0008380 [RNA splicing]
GO:0008610 [lipid biosynthetic process]
GO:0010467 [gene expression]
GO:0010498 [proteasomal protein catabolic process]
GO:0016020 [membrane]
GO:0016567 [protein ubiquitination]
GO:0016607 [nuclear speck]
GO:0016874 [ligase activity]
GO:0034450 [ubiquitin-ubiquitin ligase activity]
GO:0034613 [cellular protein localization]
GO:0035861 [site of double-strand break]
GO:0042802 [identical protein binding]
GO:0045665 [negative regulation of neuron differentiation]
GO:0048026 [positive regulation of mRNA splicing, via spliceosome]
GO:0048711 [positive regulation of astrocyte differentiation]
GO:0061630 [ubiquitin protein ligase activity]
GO:0070534 [protein K63-linked ubiquitination]
GO:0071006 [U2-type catalytic step 1 spliceosome]
GO:0071013 [catalytic step 2 spliceosome]
GO:0072422 [signal transduction involved in DNA damage checkpoint]
Show all
 504 aa
55.2 kDa 		No 0
PRPF19-002
ENSP00000446259
ENST00000546152
F5H2I0 [Direct mapping]
Pre-mRNA-processing factor 19
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000349 [generation of catalytic spliceosome for first transesterification step]
GO:0071006 [U2-type catalytic step 1 spliceosome]
Show all
 104 aa
11.4 kDa 		No 0
PRPF19-003
ENSP00000440266
ENST00000541371
F5GY56 [Direct mapping]
Pre-mRNA-processing factor 19
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000349 [generation of catalytic spliceosome for first transesterification step]
GO:0004842 [ubiquitin-protein transferase activity]
GO:0005515 [protein binding]
GO:0016567 [protein ubiquitination]
GO:0071006 [U2-type catalytic step 1 spliceosome]
Show all
 252 aa
27.7 kDa 		No 0
PRPF19-006
ENSP00000443031
ENST00000540473
H0YGF3 [Direct mapping]
Pre-mRNA-processing factor 19
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000349 [generation of catalytic spliceosome for first transesterification step]
GO:0071006 [U2-type catalytic step 1 spliceosome]
Show all
 45 aa
5.3 kDa 		No 0
PRPF19-007
ENSP00000445435
ENST00000535326
H0YGZ5 [Direct mapping]
Pre-mRNA-processing factor 19
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000349 [generation of catalytic spliceosome for first transesterification step]
GO:0005515 [protein binding]
GO:0071006 [U2-type catalytic step 1 spliceosome]
Show all
 119 aa
13.2 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.